Platelet–Leukocyte Hybrid Membrane‐Coated Immunomagnetic Beads for Highly Efficient and Highly Specific Isolation of Circulating Tumor Cells,Advanced Functional Materials

当前位置： X-MOL 学术 › Adv. Funct. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Platelet–Leukocyte Hybrid Membrane‐Coated Immunomagnetic Beads for Highly Efficient and Highly Specific Isolation of Circulating Tumor Cells
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2018-07-13 , DOI: 10.1002/adfm.201803531
Lang Rao ₁ , Qian-Fang Meng ₁ , Qinqin Huang ₁ , Zixiang Wang ₁ , Guang-Tao Yu ₂ , Andrew Li ₃ , Weijie Ma ₄ , Nangang Zhang ₅ , Shi-Shang Guo ₁ , Xing-Zhong Zhao ₁ , Kan Liu _{5,

6} , Yufeng Yuan ₄ , Wei Liu _{1,

4,

5}

Affiliation

Biomimetic cell‐membrane‐camouflaged nanoparticles with desirable features have been widely used for various biomedical applications. However, the current research focuses on single cell membrane coating and using multiple cell membranes for nanoparticle functionalization is still challenging. In this work, platelet (PLT) and leukocyte (WBC) membranes are fused, PLT–WBC hybrid membranes are coated onto magnetic beads, and then their surface is modified with specific antibodies. The resulting PLT–WBC hybrid membrane‐coated immunomagnetic beads (HM‐IMBs) inherit enhanced cancer cell binding ability from PLTs and reduce homologous WBC interaction from WBCs, and are further used for highly efficient and highly specific isolation of circulating tumor cells (CTCs). By using spiked blood samples, it is found that, compared with commercial IMBs, the cell separation efficiency of HM‐IMBs is improved to 91.77% from 66.68% and the cell purity is improved to 96.98% from 66.53%. Furthermore, by using the HM‐IMBs, highly pure CTCs are successfully identified in 19 out of 20 clinical blood samples collected from breast cancer patients. Finally, the robustness of HM‐IMBs is verified in downstream CTC analysis such as the detection of PIK3CA gene mutations. It is anticipated that this novel hybrid membrane coating strategy will open new possibilities for overcoming the limitations of current theranostic platforms.

中文翻译：

血小板-白细胞杂交膜包被的免疫磁珠用于循环肿瘤细胞的高效和高特异性分离

具有理想功能的仿生细胞膜伪装纳米颗粒已广泛用于各种生物医学应用中。然而，当前的研究集中在单细胞膜涂层上，使用多细胞膜进行纳米粒子功能化仍然具有挑战性。在这项工作中，将血小板（PLT）和白细胞（WBC）膜融合，将PLT–WBC杂化膜包被在磁珠上，然后用特异性抗体修饰它们的表面。所得的PLT-WBC混合膜包被的免疫磁珠（HM-IMB）继承了PLT增强的癌细胞结合能力，并减少了WBC的同源WBC相互作用，并进一步用于高效，高特异性地分离循环肿瘤细胞（CTC）。通过使用加标的血液样本，发现与商用IMB相比，HM-IMBs的细胞分离效率从66.68％提高到91.77％，细胞纯度从66.53％提高到96.98％。此外，通过使用HM-IMB，在从乳腺癌患者收集的20份临床血液样本中，有19份成功鉴定出高纯度的CTC。最后，HM-IMB的鲁棒性在下游CTC分析中得到了验证，例如检测PIK3CA基因突变。可以预期，这种新颖的混合膜包衣策略将为克服当前治疗学平台的局限性开辟新的可能性。

更新日期：2018-07-13

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11