Intrinsically disordered proteins (IDPs) are getting more and more interest of the scientific community. Nuclear magnetic resonance (NMR) is often a technique of choice for these studies, as it provides atomic-resolution information on structure, dynamics and interactions of IDPs. Nonetheless, NMR spectra of IDPs are typically extraordinary crowded, comparing to those of structured proteins. To overcome this problem, high-dimensional NMR experiments can be used, which allow for a better peak separation. In the present review different aspects of such experiments are discussed, from data acquisition and processing to analysis, focusing on experiments for resonance assignment.

中文翻译：

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用于内在无序蛋白质研究的高维核磁共振方法

内在无序蛋白质 (IDP) 越来越受到科学界的关注。核磁共振 (NMR) 通常是这些研究的首选技术，因为它提供有关 IDP 结构、动力学和相互作用的原子分辨率信息。尽管如此，与结构化蛋白质的核磁共振谱相比，IDP 的核磁共振谱通常非常拥挤。为了克服这个问题，可以使用高维 NMR 实验，从而实现更好的峰分离。在本综述中，讨论了此类实验的不同方面，从数据采集和处理到分析，重点是共振分配实验。