Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice†,Photochemical & Photobiological Sciences

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Irradiation with 310 nm and 340 nm ultraviolet light-emitting-diodes can improve atopic dermatitis-like skin lesions in NC/Nga mice†
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1039/c8pp00063h
Tae-Rin Kwon _{1,

2,

3,

4} , Jong Hwan Kim _{1,

2,

3,

4,

5} , Ji-Yeon Hong _{1,

2,

3,

4,

5} , Joon Seok _{1,

2,

3,

4,

5} , Jae Min Kim _{1,

2,

3,

4,

5} , Dong-Ho Bak _{1,

2,

3,

4,

5} , Mi-Ji Choi _{1,

2,

3,

4,

5} , Seok Kyun Mun _{2,

3,

4,

6} , Chan Woong Kim _{2,

3,

4,

7} , Beom Joon Kim _{1,

2,

3,

4,

5}

Affiliation

Ultraviolet (UV) light produces an immunomodulatory effect on the skin and is widely used for the treatment of chronic inflammatory skin diseases. UV light emitting diodes (UV-LEDs) are a new and promising source of UV radiation. However, their mechanism of action remains largely unknown. In this study, we tested the safety and effectiveness of UV-LED irradiation for the treatment of atopic dermatitis (AD) in an NC/Nga mouse model. Mice were divided into seven groups of eight mice each. Application of Dermatophagoides farinae (Df) extract ointment for four weeks induced AD-like skin lesions. Subsequently, the mice were exposed to UV-LEDs, narrow band UVB, or UVA irradiation three times per week. We assessed the immunosuppressive effects of 310 nm (50 mJ cm⁻²) and 340 nm (5 J cm⁻²) UV-LED irradiation. Histological analyses using hematoxylin–eosin, toluidine blue, and immunohistochemical staining were performed. In addition, the serum levels of IgE, inflammatory cytokines and chemokines were measured using enzyme-linked immunosorbent assays (ELISAs). UV-LED irradiation significantly alleviated AD-like skin symptoms, including edema, erythema, dryness, and itching, by modulating Th1 and Th2 responses, transepidermal water loss (TEWL), and scratching behavior in NC/Nga mice. These results suggest that UV-LEDs can improve the treatment of inflammatory skin diseases.

中文翻译：

用310 nm和340 nm紫外发光二极管进行辐照可以改善NC / Nga小鼠的特应性皮炎样皮肤病变†

紫外线（UV）对皮肤产生免疫调节作用，被广泛用于治疗慢性炎症性皮肤病。紫外线发光二极管（UV-LED）是一种新的有希望的紫外线辐射源。但是，它们的作用机理仍然未知。在这项研究中，我们测试了UV / LED辐射在NC / Nga小鼠模型中治疗特应性皮炎（AD）的安全性和有效性。将小鼠分成七组，每组八只小鼠。应用法氏皮肤癣菌（Df）提取物软膏持续四周可诱发AD样皮肤病变。随后，每周将小鼠暴露于UV-LED，窄带UVB或UVA照射下3次。我们评估了310 nm（50 mJ cm ^-2）和340 nm（5 J cm^-2）UV-LED照射。使用苏木精-曙红，甲苯胺蓝和免疫组化染色进行组织学分析。另外，使用酶联免疫吸附测定（ELISA）测量血清IgE，炎性细胞因子和趋化因子的水平。UV-LED辐射可通过调节Th1和Th2反应，经皮失水（TEWL）和NC / Nga小鼠的抓挠行为来显着缓解AD样皮肤症状，包括水肿，红斑，干燥和瘙痒。这些结果表明UV-LED可以改善炎性皮肤疾病的治疗。

更新日期：2018-07-13

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