Investigation of the iron oxidation state in ovarian cancer using synchrotron micro X-ray absorption near edge structure – preliminary results,Journal of Analytical Atomic Spectrometry

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Investigation of the iron oxidation state in ovarian cancer using synchrotron micro X-ray absorption near edge structure – preliminary results
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1039/c8ja00140e
D. M. Krauze _{1,

2,

3,

4,

5} , M. Grzelak _{1,

2,

3,

4,

5} , P. Wróbel _{1,

2,

3,

4,

5} , G. Veronesi _{6,

7,

8,

9} , H. Castillo _{8,

9,

10} , L. Chmura _{4,

5,

11,

12,

13} , D. Adamek _{4,

5,

11,

12,

13} , R. Jach _{4,

5,

12,

13,

14} , M. Lankosz _{1,

2,

3,

4,

5}

Affiliation

Iron as a trace element plays a role in regulating the homeostasis of the human body and therefore the concentration of iron in the body must be carefully monitored. Both deficiency and overload of iron could have a serious effect on health. Iron in excess can be carcinogenic in different ways. As a catalyst of Fenton's reaction it leads to the creation of reactive oxygen species which causes oxidative stress and DNA damage, among other things. Iron can also reduce the response of the immune system to cancer and induce increased cancer cell proliferation. Ovarian cancer is one of the most common neoplasms in women and has a low survival rate. For this reason, there is strong motivation to deepen the understanding of the processes that lead to the disease and take place in ovarian cancer tissue. In this study, cryo micro X-ray absorption near edge structure spectroscopy was used to analyse the oxidation state of iron in ovarian cancer samples. For calibration, organic and inorganic reference samples containing Fe in different oxidation states were used. To determine the trace element in the neoplasm samples, the pre-edge region, white line and absorption edge position of Fe K-edge XANES spectra were examined and compared to those of the recorded reference materials. A comparison of these XANES spectral features shows the existence of differences in the iron oxidation state between the neoplasm samples and suggests the possibility that a combination of iron ions can occur in one sample. Overall, further investigations are required to make a better comparison and determine what organic compounds are contained in the samples and possibly carry out a statistical analysis.

中文翻译：

利用同步加速器X射线近边缘结构吸收研究卵巢癌中铁的氧化态–初步结果

铁作为微量元素在调节人体的体内平衡中起着重要的作用，因此必须仔细监测人体中铁的浓度。铁的缺乏和过量都可能对健康产生严重影响。过量的铁可能以不同的方式致癌。作为Fenton反应的催化剂，它导致了活性氧的产生，这引起氧化应激和DNA损伤等。铁还可以减少免疫系统对癌症的反应，并诱导癌细胞增殖增加。卵巢癌是女性最常见的肿瘤之一，生存率很低。因此，强烈希望加深对导致该疾病并在卵巢癌组织中发生的过程的了解。在这项研究中，冷冻微X射线吸收近边缘结构光谱用于分析卵巢癌样品中铁的氧化态。为了校准，使用了含有不同氧化态的铁的有机和无机参考样品。为了确定肿瘤样品中的痕量元素，检查了Fe K边缘XANES光谱的前边缘区域，白线和吸收边缘位置，并将其与记录的参考材料进行比较。对这些XANES光谱特征的比较表明，在肿瘤样品之间存在铁氧化态差异，并暗示在一个样品中可能发生铁离子结合的可能性。全面的，

更新日期：2018-07-13

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