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Top-down Approach to Produce Protein Functionalized and Highly Thermally Stable Cellulose Fibrils
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1021/acs.biomac.8b00831 Franck Quero 1 , Genesis Opazo 1 , Yadong Zhao 2 , Aymeric Feschotte-Parazon 1 , Jeimy Fernandez 1 , Abraham Quintro 1 , Marcos Flores 3
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1021/acs.biomac.8b00831 Franck Quero 1 , Genesis Opazo 1 , Yadong Zhao 2 , Aymeric Feschotte-Parazon 1 , Jeimy Fernandez 1 , Abraham Quintro 1 , Marcos Flores 3
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Protein-functionalized cellulose fibrils, having various amounts of covalently bonded proteins at their surface, were successfully extracted from the tunic of Pyura chilensis tunicates using successive alkaline extractions. Pure cellulose fibrils were also obtained by further bleaching and were used as reference material. Extraction yields of protein-functionalized cellulose fibrils were within the range of 62–76% by weight based on the dry initial tunic powder. Fourier-transform infrared and Raman spectroscopy confirmed the preservation of residual protein at the surface of cellulose fibrils, which was then quantified by X-ray photoelectron spectroscopy. The protein-functionalized cellulose fibrils were found to have relatively high crystallinity and their cellulose I crystalline structure was preserved upon applying alkaline treatments. The extracted cellulosic materials were found to be constituted of fibrils having a ribbon-like morphology with widths ranging from ∼30 nm up to ∼400 nm. These protein-functionalized cellulose fibrils were found to have outstanding thermal stability with one of them having onset and peak degradation temperatures of ∼350 and 374 °C, respectively. These values were found to be 24 and 41 °C higher than for bleached cellulose.
中文翻译：
自上而下的方法来生产蛋白质功能化和高度热稳定的纤维素原纤维
从Pyura chilensis的外衣中成功地提取了在表面具有各种共价键合蛋白质的蛋白质功能化纤维素原纤维。使用连续的碱提取进行包被。也可以通过进一步漂白获得纯的纤维素原纤维，并将其用作参考材料。蛋白质功能化纤维素原纤维的提取率以干燥的中山药粉为基准，为62-76％（重量）。傅里叶变换红外光谱和拉曼光谱证实了纤维素原纤维表面残留蛋白的保留，然后通过X射线光电子能谱对其进行了定量。发现蛋白质官能化的纤维素原纤维具有相对较高的结晶度，并且在进行碱处理后其纤维素I的晶体结构得以保留。发现提取的纤维素材料由具有带状形态的原纤维构成，其宽度为约30nm至约400nm。发现这些蛋白质官能化的纤维素原纤维具有出色的热稳定性，其中之一的起始降解温度和峰值降解温度分别约为350和374°C。发现这些值比漂白纤维素高24和41℃。
更新日期：2018-07-13
中文翻译：
自上而下的方法来生产蛋白质功能化和高度热稳定的纤维素原纤维
从Pyura chilensis的外衣中成功地提取了在表面具有各种共价键合蛋白质的蛋白质功能化纤维素原纤维。使用连续的碱提取进行包被。也可以通过进一步漂白获得纯的纤维素原纤维，并将其用作参考材料。蛋白质功能化纤维素原纤维的提取率以干燥的中山药粉为基准，为62-76％（重量）。傅里叶变换红外光谱和拉曼光谱证实了纤维素原纤维表面残留蛋白的保留，然后通过X射线光电子能谱对其进行了定量。发现蛋白质官能化的纤维素原纤维具有相对较高的结晶度，并且在进行碱处理后其纤维素I的晶体结构得以保留。发现提取的纤维素材料由具有带状形态的原纤维构成，其宽度为约30nm至约400nm。发现这些蛋白质官能化的纤维素原纤维具有出色的热稳定性，其中之一的起始降解温度和峰值降解温度分别约为350和374°C。发现这些值比漂白纤维素高24和41℃。
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