A Self-Contained Chemiluminescent Lateral Flow Assay for Point-of-Care Testing,Analytical Chemistry

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A Self-Contained Chemiluminescent Lateral Flow Assay for Point-of-Care Testing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1021/acs.analchem.8b01543
Jinqi Deng _{1,

2,

3} , Mingzhu Yang ₁ , Jing Wu ₁ , Wei Zhang _{1,

2} , Xingyu Jiang _{1,

2}

Affiliation

Immunoassays whose readouts rely on chemiluminescence are increasingly useful for a broad range of analytical applications, but they are rarely made into point-of-care (POC) format because of the complex reagents required (some reagents have to be stored in low temperatures, and some reagents have to be freshly made right before the assay). This study reports a self-contained chemiluminescent lateral flow assay (CLFA), which prestores all necessary reagents. This CLFA contains three parts: the normal lateral flow assay (LFA) strip, the chemiluminescence substrate pad, and the polycarbonate (PC) holder. On the LFA strip, we simultaneously labeled horseradish peroxidase (HRP) and antibody on the gold nanoparticles (AuNPs) for the conjugate pad. For the substrate pad, we used sodium perborate as the oxidant and lyophilized the chemiluminescence substrate on the glass fiber, which allows long-term storage. After the transfer of substrate from the substrate pad to the nitrocellulose (NC) membrane, we captured the chemiluminescence signal for the quantification of the targets. The HRP on the AuNPs can amplify the chemiluminescence signal efficiently. We used this CLFA system to detect both macromolecules and small molecules successfully. This self-contained and easily processable device is exceedingly appropriate for rapid detection and is a convenient platform for POC testing.

中文翻译：

用于现场检测的自包含化学发光侧向流动测定

读数依赖化学发光的免疫测定法在各种分析应用中越来越有用，但由于所需的试剂复杂，因此很少将其制成即时点（POC）格式（某些试剂必须在低温下保存，并且一些试剂必须在测定前立即重新制备）。这项研究报告了一种独立的化学发光侧向流动测定法（CLFA），该测定法可预先存储所有必要的试剂。此CLFA包含三个部分：正常侧向流动检测（LFA）试纸条，化学发光底物垫和聚碳酸酯（PC）固定器。在LFA试纸上，我们同时在偶联垫的金纳米颗粒（AuNPs）上标记了辣根过氧化物酶（HRP）和抗体。对于基板垫，我们使用过硼酸钠作为氧化剂，并将玻璃纤维上的化学发光底物冻干，从而可以长期保存。将底物从底物垫转移到硝酸纤维素（NC）膜后，我们捕获了化学发光信号，用于定量目标。AuNPs上的HRP可以有效地放大化学发光信号。我们使用此CLFA系统成功检测了大分子和小分子。这种设备齐全且易于处理的设备非常适合快速检测，并且是POC测试的便捷平台。AuNPs上的HRP可以有效地放大化学发光信号。我们使用此CLFA系统成功检测了大分子和小分子。这种设备齐全且易于处理的设备非常适合快速检测，并且是POC测试的便捷平台。AuNPs上的HRP可以有效地放大化学发光信号。我们使用此CLFA系统成功检测了大分子和小分子。这种设备齐全且易于处理的设备非常适合快速检测，并且是POC测试的便捷平台。

更新日期：2018-07-13

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