Engineering Organelle-Specific Molecular Viscosimeters Using Aggregation-Induced Emission Luminogens for Live Cell Imaging,Analytical Chemistry

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Engineering Organelle-Specific Molecular Viscosimeters Using Aggregation-Induced Emission Luminogens for Live Cell Imaging
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-07-13 00:00:00 , DOI: 10.1021/acs.analchem.8b02940
Wen Chen ₁ , Cai Gao ₁ , Xianjun Liu ₁ , Feng Liu ₁ , Fenglin Wang ₁ , Li-Juan Tang ₁ , Jian-Hui Jiang ₁

Affiliation

Subcellular viscosity is essential for cell functions and may indicate its physiological status. We screen two fluorescent probes by engineering tetraphenylethene (TPE) for measuring viscosity in mitochondria and lysosomes, respectively. These two probes are only weakly emissive in nonviscous medium and the emission signals are greatly enhanced in viscous medium due to the restriction of intramolecular motion. The presence of pyridium has endowed one probe with mitochondrial specificity, while the presence of indole ring has granted the other probe with lysosome-targeting ability. Their optical properties are characterized in vitro and their applications in imaging viscosity variations in mitochondria and lysosomes are also demonstrated in living cells under different stimulated processes. In addition, an increase in both mitochondrial and lysosomal viscosity during mitophagy was revealed for the first time with our probes. To our knowledge, this is the first time that TPE is engineered to be fluorescent molecular viscosimeters that possess desirable aqueous solubility, red-shifted emission, and organelle specificity.

中文翻译：

使用聚集诱导的发射致发光剂工程化细胞器的特定分子粘度计，用于活细胞成像

亚细胞粘度对于细胞功能至关重要，并可能表明其生理状态。我们通过工程四苯乙烯（TPE）筛选了两个荧光探针，分别用于测量线粒体和溶酶体中的粘度。这两种探针仅在非粘性介质中微弱发射，并且由于分子内运动的限制，在粘性介质中的发射信号大大增强。吡啶的存在赋予了一种探针线粒体特异性，而吲哚环的存在赋予了另一种探针以溶酶体靶向的能力。它们的光学特性在体外得到表征在活细胞中，在不同的刺激过程下，其在线粒体和溶酶体粘度变化成像中的应用也得到了证实。另外，我们的探针首次揭示了线粒体吞噬过程中线粒体和溶酶体粘度的增加。据我们所知，这是TPE首次被设计成具有理想的水溶性，红移发射和细胞器特异性的荧光分子粘度计。

更新日期：2018-07-13

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