Abstract Working at high values of lignocellulosic Dry Matter (DM), as wheat straw, increases the reaction medium viscosity, making the mixing inefficient with the traditional agitators. Batch and fed-batch tests were conducted using different impellers: i) inclined blades, ii) marine impeller, iii) anchor, iv) paravisc and v) double helical impeller. Inclined blades appeared an inadequate device for batch and fed-batch tests. On contrary, double helical impellers and anchor gave optimal performances. An alternative to improve the reactor’s rheology is the modification of the feeding strategy. A particular fed-batch strategy allowed keeping low the reaction medium viscosity by a gradual increasing of the DM content in the reactor. In this way, three main benefits were achieved: i) a very good performances in terms of glucose concentration (85 g/L), ii) a strong reduction of the energetic consumption compared to batch test and iii) the adoption of a simple mixing devise.

中文翻译：

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高木质纤维素含量下的酶促水解：优化混合系统几何形状和提高葡萄糖浓度的分批补料策略

摘要 在高含量的木质纤维素干物质 (DM) 下工作，如麦秆，会增加反应介质的粘度，使传统搅拌器的混合效率低下。使用不同的叶轮进行分批和补料分批测试：i) 倾斜叶片，ii) 船用叶轮，iii) 锚，iv) paravisc 和 v) 双螺旋叶轮。倾斜叶片对于分批和补料分批测试来说似乎是一个不合适的设备。相反，双螺旋叶轮和锚提供最佳性能。改善反应器流变性的另一种方法是修改进料策略。特定的分批补料策略允许通过逐渐增加反应器中的 DM 含量来保持低反应介质粘度。通过这种方式，实现了三个主要好处：i) 在葡萄糖浓度 (85 g/L) 方面具有非常好的性能，