We report a benzo[a]phenoxazine-based far red-emitting probe which selectively senses reduced nicotinamide adenine dinucleotide (NADH) and its phosphate ester NADPH, the most indispensable coenzymes found in all living cells, with “turn-off” fluorescence response at 650 nm. The original probe can be regenerated quantitatively through oxidation using hypochlorous acid, a reactive oxygen species, which was accompanied by restored fluorescence at 650 nm. This probe was also applied for fluorescence imaging of NADH in HeLa cells.

中文翻译：

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使用基于苯并[ a ]吩恶嗪的远红光染料对氧化还原物质NAD（P）H和HOCl进行双重探测

我们报道了一种基于苯并[ a ]吩恶嗪的远红色探针，该探针选择性地感测还原的烟酰胺腺嘌呤二核苷酸（NADH）及其磷酸酯NADPH，这是在所有活细胞中发现的最不可缺少的辅酶，其“关闭”荧光响应为650纳米 原始探针可以通过使用次氯酸（一种活性氧）进行氧化而定量再生，并伴有在650 nm处恢复的荧光。该探针还用于HeLa细胞中NADH的荧光成像。