Copper (Cu) pollution in aquaculture ponds poses substantial ecological threats. Most phytoremediation studies deal with the efforts of removing Cu from water, but seldom, such endeavors are validated by suitable bioassays. The present study undertook a two-pronged effort to remediate Cu by phytoextraction with an aquatic macrophyte, Eichhornia crassipes, and establish the efficacy of such endeavors by Clarias batrachus bioassay. For phytoextraction trials, E. crassipes was exposed to Cu concentration 0, 5, 10, 15 and 20 mg L^-1 in Hoagland solution for 21 days. The highest uptake of 2168 µg g^-1 dw was at 10 mg L^-1 Cu exposure, and efficient root to leaf translocation was seen for 5-10 mg Cu L^-1. For these doses, there was 55-57% decline in Cu from test waters. We evaluated morphological, physiological and biochemical response of plants towards Cu stress to gauge its phytomediation capacity. For bioassays, fish were reared for 7 days in phytoremediated Cu doses of 5 and 10 mg L^-1. The accumulation of Cu followed the pattern: kidney>liver>gill>muscle. Fish muscle accumulated 21.8-27.0 µg Cu g^-1 dw after 7d, however, for E. crassipes remediated doses, muscle accumulated 8.2-10.9 µg Cu g^-1 dw, which was within the safe levels of Cu in edible tissues. Metal doses declined protein contents and augmented malondialdehyde, superoxide dismutase, catalase and peroxidase concentrations in tissues. Although their concentrations in remediated groups failed to reach the levels of control fish, significant recovery in these parameters were observed. The results pointed towards the efficacy of Cu phytoextration by E. crassipes.

水产养殖池塘中的铜（Cu）污染构成了严重的生态威胁。大多数植物修复研究都涉及从水中去除Cu的努力，但很少有此类努力通过适当的生物测定法得到验证。本研究进行了两方面的努力，以水生大型植物凤眼凤梨通过植物提取来补救铜，并通过苦竹（Clarias batrachus）生物测定法建立了这种努力的功效。对于植物提取试验，在Hoagland溶液中，将十字花肠杆菌暴露于Cu浓度为0、5、10、15和20 mg L ^-1的情况下持续21天。2168 µg g ^-1 dw的最高吸收量为10 mg L ^-1铜暴露和有效的根到叶易位对于5-10 mg Cu L ^-1可见。对于这些剂量，测试水中的铜含量下降了55-57％。我们评估了植物对铜胁迫的形态，生理和生化反应，以评估其植磷能力。为了进行生物测定，将鱼用经过植物修复的铜剂量分别为5和10 mg L ^-1饲养7天。Cu的积累遵循以下模式：肾脏>肝脏> g>肌肉。鱼肉在7天后累积了21.8-27.0 µg Cu g ^-1 dw，但是，对于E. crassipes修复剂量，肌肉累积了8.2-10.9 µg Cu g ^-1dw，在食用组织中的铜的安全含量之内。金属剂量降低了蛋白质含量，并增加了组织中的丙二醛，超氧化物歧化酶，过氧化氢酶和过氧化物酶浓度。尽管它们在修复组中的浓度未能达到对照鱼的水平，但观察到这些参数的显着恢复。该结果表明了由景天肠杆菌（E. crassipes）对铜进行植物灭绝的功效。