Tunable Enzymatically Cross‐Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration,Advanced Healthcare Materials

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Tunable Enzymatically Cross‐Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-07-12 , DOI: 10.1002/adhm.201800186
Cristiana R. Carvalho _{1,

2,

3} , João B. Costa _{1,

2,

3} , Alain da Silva Morais _{1,

2} , Rita López-Cebral _{1,

2,

3} , Joana Silva-Correia _{1,

2} , Rui L. Reis _{1,

2,

3} , J. Miguel Oliveira _{1,

2,

3}

Affiliation

Hollow tubular conduits (TCs) with tunable architecture and biological properties are in great need for modulating cell functions and drug delivery in guided tissue regeneration. Here, a new methodology to produce enzymatically cross‐linked silk fibroin TCs is described, which takes advantage of the tyrosine groups present in silk structure that are known to allow the formation of a covalently cross‐linked hydrogel. Three different processing methods are used as a final step to modulate the properties of the silk‐based TCs. This approach allows to virtually adjust any characteristic of the final TCs. The final microstructure ranges from a nonporous to a highly porous network, allowing the TCs to be selectively porous to 4 kDa molecules, but not to human skin fibroblasts. Mechanical properties are dependent both on the processing method and thickness of the TCs. Bioactivity is observed after 30 days of immersion in simulated body fluid only for the TCs submitted to a drying processing method (50 °C). The in vivo study performed in mice demonstrates the good biocompatibility of the TCs. The enzymatically cross‐linked silk fibroin TCs are versatile and have adjustable characteristics that can be exploited in a variety of biomedical applications, particularly in guidance of peripheral nerve regeneration.

中文翻译：

可调节的酶交联丝素蛋白管形导管，用于引导组织再生。

具有可调结构和生物学特性的空心管状导管（TC）非常需要在引导的组织再生中调节细胞功能和药物输送。在此，描述了一种生产酶促交联的丝素蛋白TC的新方法，该方法利用了存在于丝绸结构中的酪氨酸基团，这些酪氨酸基团已知可以形成共价交联的水凝胶。最后三种方法是调制基于丝的TC的属性。这种方法允许虚拟调整最终TC的任何特性。最终的微观结构从无孔网络到高度多孔的网络，使TC选择性地渗透到4 kDa分子，但不能渗透到人皮肤成纤维细胞。机械性能取决于TC的加工方法和厚度。浸入模拟体液中30天后，仅对经过干燥处理方法（50°C）的TC观察到生物活性。在小鼠中进行的体内研究证明了TC的良好生物相容性。酶促交联的丝素蛋白TC具有多种用途，并具有可调节的特性，可用于多种生物医学应用，特别是在指导周围神经再生方面。

更新日期：2018-07-12

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