The development of mirror-image biology systems faces a crucial barrier of lacking an L-DNA sequencing technique. Here, we developed a practical method for sequencing mirror-image DNA by adopting the Maxam-Gilbert sequencing approach, through which specific nucleobases in an end-labeled L-DNA are cleaved by achiral chemicals. This technique may facilitate the therapeutic application of nuclease-resistant L-aptamer drugs, and bring the vision of building an alternative, mirror-image self-replicating system closer to reality.

中文翻译：

---

化学镜像DNA测序

镜像生物学系统的发展面临缺少L-DNA测序技术的关键障碍。在这里，我们通过采用Maxam-Gilbert测序方法开发了一种实用的方法，用于对镜像DNA进行测序，通过该方法，末端标记的L-DNA中的特定核碱基可被非手性化学品裂解。该技术可以促进抗核酸酶的L-适体药物的治疗应用，并使构建替代的镜像自我复制系统的愿景更接近现实。