High-speed imaging is an indispensable tool in today's scientific research, industry, and energy because it enables blur-free observation and monitoring of fast transient dynamics. It is commonly used for various applications, including sports, manufacturing, and fusion science, in which slow-motion analysis is conducted to elucidate their dynamics. On the other hand, the past several years have seen another and radically new application of high-speed imaging, namely single-cell analysis—the study of individual biological cells among populations for identifying cell-to-cell differences and elucidating cellular heterogeneity invisible to population-averaged measurements. This exciting application has been made possible by exploiting high-speed imaging's capability of acquiring information-rich images at high frame rates to provide a snapshot library of numerous cells in a short duration of time (with one cell per frame). In this article, we review the principles of several unique high-speed imaging methods for microscopy and their emerging application to large-scale single-cell analysis.

高速成像是当今科学研究，工业和能源中必不可少的工具，因为它可以实现快速瞬态动态的无模糊观察和监视。它通常用于各种应用程序，包括体育，制造和融合科学，在其中进行慢动作分析以阐明其动力学。另一方面，在过去的几年中，高速成像技术又有了另一个全新的应用，即单细胞分析，即研究人群中单个生物细胞以鉴定细胞之间的差异并阐明细胞不可见的异质性。总体平均测量。通过开发高速成像技术，使这一激动人心的应用成为可能。能够以高帧速率获取信息丰富的图像，从而在短时间内提供大量单元的快照库（每帧一个单元）。在本文中，我们回顾了几种独特的显微镜高速成像方法的原理及其在大规模单细胞分析中的新兴应用。