Abstract Pure hydroxyapatite (HA), HA enriched in Mg2+ ions (Mg-HA) and SiO44- ions (Si-HA), as well as a co-substituted sample (MgSi-HA), were synthesized using the precipitation method and systematically characterized according to various analytical methods. The degree of substitution was examined by inductively coupled plasma optical emission spectrometry (ICP-OES). The chemical structure of the samples was determined by mid-infrared spectroscopy (FT-IR) and solid-state nuclear magnetic resonance (ssNMR). The powder X-ray diffractometry (PXRD) method was used for the phase identification and characterization of unit cell parameters. An evaluation of the crystal morphology was carried out by transmission electron microscopy (TEM). All of the samples turned out to be cytocompatible, as confirmed by an in vitro test on the BALB/c 3T3 fibroblasts cell line. Moreover, the MgSi-HA sample was used to prepare granules based on sodium alginate and chondroitin sulphate. A new method of crosslinking using Mg2+ ions was developed. The low-porous granules obtained from this process were then characterized using scanning electron microscopy (SEM).

中文翻译：

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基于 Mg 2+ 和 SiO 4 4- 共取代纳米羟基磷灰石、海藻酸盐和硫酸软骨素的新型杂化材料，可用于生物材料工程

摘要 利用沉淀法合成了纯羟基磷灰石 (HA)、富含 Mg2+ 离子 (Mg-HA) 和 SiO44- 离子 (Si-HA) 的 HA 以及共取代样品 (MgSi-HA)，并对其进行了系统表征。根据不同的分析方法。通过电感耦合等离子体发射光谱法(ICP-OES)检查取代度。样品的化学结构由中红外光谱 (FT-IR) 和固态核磁共振 (ssNMR) 确定。粉末X射线衍射(PXRD)方法用于晶胞参数的相鉴定和表征。通过透射电子显微镜（TEM）进行晶体形态的评估。结果证明所有样品都是细胞相容的，BALB/c 3T3 成纤维细胞系的体外试验证实了这一点。此外，MgSi-HA 样品用于制备基于海藻酸钠和硫酸软骨素的颗粒。开发了一种使用 Mg2+ 离子进行交联的新方法。然后使用扫描电子显微镜 (SEM) 表征从该过程获得的低孔颗粒。