An anoxic/oxic membrane bioreactor (AO-MBR) and three anaerobic side-stream reactor (ASSR) coupled MBRs (ASSR-MBR) were operated to investigate the effects of hydraulic retention time of ASSR (HRT_A) and to elucidate sludge reduction mechanisms in ASSR-MBRs. Increasing HRT_A from 3.3 to 6.6 h improved nitrogen removal, and enhanced sludge reduction from 8.0% to 40.9% in ASSR-MBR. The sludge decay coefficient was 0.0221 d⁻¹ in MBRs, and 0.0231–0.0345 d⁻¹ in ASSRs. The measured lysis rate coefficient of heterotrophic biomass was 0.083–0.112 d⁻¹ in MBRs and 0.079–0.111 d⁻¹ in ASSRs. The hydrolysis rate coefficient of inactive particulate organic matters (POMs) in ASSRs significantly exceeded that in the MBR. At HRT_A of 6.6 h, POMs hydrolysis in ASSR (38.6%) is the dominant route of sludge reduction, and cell lysis occurred principally in aerobic tanks. Illumina-MiSeq sequencing showed ASSR-MBRs enriched hydrolytic and fermentative bacteria, and confirmed that anaerobic hydrolysis contributed most to sludge reduction.

运行缺氧/氧化膜生物反应器（AO-MBR）和三个厌氧侧流反应器（ASSR）耦合的MBR（ASSR-MBR），以研究ASSR（HRT _A）的水力停留时间的影响并阐明污泥减少的机理在ASSR-MBR中。在ASSR-MBR中，将HRT _A从3.3 h增加到6.6 h可以改善脱氮效果，并将污泥减少量从8.0％提高到40.9％。污泥衰减系数为0.0221 d ^-1中的MBR，及0.0231-0.0345 d ^-1在ASSRs。在MBRs中测得的异养生物质的裂解速率系数为0.083–0.112 d ^-1和0.079–0.111 d ^-1在ASSR中。ASSR中非活性颗粒有机物（POM）的水解速率系数大大超过了MBR中的水解速率系数。在6.6 h的HRT _A下，ASSR中的POMs水解（38.6％）是污泥减少的主要途径，并且细胞裂解主要发生在好氧池中。Illumina-MiSeq测序显示ASSR -MBRs富集水解和发酵细菌，并证实厌氧水解对污泥的减少贡献最大。