This work focuses to investigate the impact of lignin content on chemical compositions, crystallinity, surface characterizations, cellulase adsorption profiles and hydrolysability of Hybrid Pennisetum (HP) after alkaline sulfite pretreatment (ASP). For the HP with lower lignin content, the increase of the cellulose content by ASP was more obvious than raw HP. ASP decreased total lignin content and surface lignin content of HP substrates. HP with lower lignin content (e.g., ∼15%) is suitable for ASP, because a pretty perfect glucose yield (91%) was obtained using a low dosage of enzyme loadings (5 FPU of cellulases/g dry matter). The study provides a potential strategy to efficiently produce platform sugars from HP with reduced lignin content, indicating the importance of reduction HP lignin content properly by breeding or transgenesis programs. The work could also help elucidate the mechanism of alkaline sulfite pretreatment for efficient production of fermentable sugars from lignocelluloses.

这项工作致力于研究木质素含量对碱性亚硫酸盐预处理（ASP）后杂交狼尾草（HP）的化学成分，结晶度，表面特性，纤维素酶吸附曲线和水解性的影响。对于木质素含量较低的HP，ASP处理后纤维素含量的增加比原始HP更为明显。ASP降低了HP基材的总木质素含量和表面木质素含量。木质素含量较低（例如，约15％）的HP适用于ASP，因为使用低剂量的酶负载量（纤维素酶5 FPU / g干物质）可获得相当理想的葡萄糖产率（91％）。这项研究提供了一种潜在的策略，可以有效地从HP中生产木质素含量降低的平台糖，这表明通过育种或转基因程序适当降低HP木质素含量的重要性。