Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities,ACS Central Science

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Oligomer Formation Propensities of Dimeric Bundle Peptides Correlate with Cell Penetration Abilities
ACS Central Science ( IF 12.7 ) Pub Date : 2018-07-10 00:00:00 , DOI: 10.1021/acscentsci.8b00262
Soonsil Hyun ₁ , Yuno Lee ₂ , Sun Mi Jin ₃ , Jane Cho ₃ , Jeemin Park ₄ , Changbong Hyeon ₂ , Key-Sun Kim ₄ , Yan Lee ₅ , Jaehoon Yu ₃

Affiliation

LK-3, an amphipathic dimeric peptide linked by two disulfide bonds, and related isomeric bundles were synthesized, and their cell penetrating abilities were investigated. The measurements using size exclusion chromatography and dynamic light scattering show that LK-3 and its isomers form cell penetrating oligomers. Calculations, performed for various types of peptide isomers, elucidate a strong correlation between the amphipathic character of dimers and cell penetration ability. The results suggest that the amphipathicities of LK-3 and related bundle dimers are responsible for their oligomerization propensities which in turn determine their cell penetrating abilities. The observations made in this study provide detailed information about the mechanism of cell uptake of LK-3 and suggest a plausible insight of the early stage of nanoparticle formation of the cell penetrating amphipathic peptides.

中文翻译：

二聚体捆绑肽的低聚物形成倾向与细胞穿透能力相关。

合成了通过两个二硫键连接的两亲性二聚体肽LK-3，以及相关的同分异构束，并研究了它们的细胞穿透能力。使用尺寸排阻色谱法和动态光散射法进行的测量表明，LK-3及其异构体形成了可穿透细胞的低聚物。对各种类型的肽异构体进行的计算阐明了二聚体的两亲特性与细胞渗透能力之间的密切相关性。结果表明，LK-3和相关的束二聚体的两亲性是其低聚倾向的原因，而低聚倾向又决定了它们的细胞穿透能力。

更新日期：2018-07-10

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