Self-Assembly of Large DNA Origami with Custom-Designed Scaffolds,ACS Applied Materials & Interfaces

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Self-Assembly of Large DNA Origami with Custom-Designed Scaffolds
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-07-10 00:00:00 , DOI: 10.1021/acsami.8b09222
Xiaoxing Chen ₁ , Qian Wang ₁ , Jin Peng ₁ , Qipeng Long ₁ , Hanyang Yu ₁ , Zhe Li ₁

Affiliation

As a milestone in DNA self-assembly, DNA origami has demonstrated powerful applications in many fields. However, the scarce availability of long single-stranded DNA (ssDNA) limits the size and sequences of DNA origami nanostructures, which in turn impedes the further development. In this study, we present a robust strategy to produce long circular ssDNA scaffold strands with custom-tailored lengths and sequences. These ssDNA products were then used as scaffolds for constructing various DNA origami nanostructures. This scalable method produces ssDNA at low cost with high purity and high yield, which can enable production of custom-designed DNA origami for various applications.

中文翻译：

带有定制设计支架的大型DNA折纸的自组装

作为DNA自组装的里程碑，DNA折纸已在许多领域得到了证明。但是，长单链DNA（ssDNA）的稀缺性限制了DNA折纸纳米结构的大小和序列，从而阻碍了进一步的发展。在这项研究中，我们提出了一个强大的策略来生产具有定制长度和序列的长圆形ssDNA支架链。然后将这些ssDNA产物用作构建各种DNA折纸纳米结构的支架。这种可扩展的方法可以低成本，高纯度和高产量生产ssDNA，从而可以生产用于各种应用的定制设计的DNA折纸。

更新日期：2018-07-10

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