This paper describes a one-step, chemical-free method to generate micropatterned in vitro neuronal networks on chemically unmodified reduced graphene oxide. The suggested method relies on infrared-based photothermal reduction of graphene oxide, which concurrently leads to the formation of submicrometer-scale surface roughness that promotes neuronal adhesion and guides neurite outgrowth. A commercially available laser source (LightScribe DVD drive) controlled by a computer software can be used to reduce graphene oxide (GO), and its repetitive scribing to a GO film brings about gradual increase and decrease in electrical conductivity and neurite guiding ability of the scribed regions, respectively. Our results also indicate that the observed adhesion-promoting and neurite guiding effect originate from the contrast in surface nanotopography, but not that in conductivity. This method is readily applicable to diverse graphene-based biomedical devices.

中文翻译：

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激光划刻还原氧化石墨烯的神经突引导

本文介绍了一种单步免化学方法，可在未经化学修饰的还原氧化石墨烯上生成微模式的体外神经元网络。建议的方法依赖于基于红外的氧化石墨烯光热还原，这同时导致亚微米级表面粗糙度的形成，从而促进神经元粘附并引导神经突向外生长。可以使用由计算机软件控制的市售激光源（LightScribe DVD驱动器）来还原氧化石墨烯（GO），并将其重复刻在GO膜上会导致划痕的电导率和神经突引导能力逐渐增加和降低。地区。我们的结果还表明，观察到的促黏附和神经突引导作用源自表面纳米形貌的对比，但在电导率上却不是。该方法易于适用于各种基于石墨烯的生物医学装置。