Mechanism of BRAF Activation through Biochemical Characterization of the Recombinant Full‐Length Protein,ChemBioChem

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Mechanism of BRAF Activation through Biochemical Characterization of the Recombinant Full‐Length Protein
ChemBioChem ( IF 2.6 ) Pub Date : 2018-08-17 , DOI: 10.1002/cbic.201800359
Nicholas Cope ₁ , Christine Candelora ₁ , Kenneth Wong ₁ , Sujeet Kumar ₁ , Haihan Nan ₁ , Michael Grasso ₂ , Borna Novak ₁ , Yana Li ₃ , Ronen Marmorstein ₂ , Zhihong Wang ₁

Affiliation

Home of the BRAF: A strategy to obtain full‐length BRAF that is significantly more active than its truncated counterpart is reported. To gain a better understanding of BRAF regulation, a detailed kinetic analysis of purified full‐length BRAF is conducted to investigate the impact of BRAF inhibitors, MEK binding, RAS binding, and heterodimerization with CRAF on kinase activity in vitro.

中文翻译：

通过重组全长蛋白质的生化表征进行BRAF活化的机制

BRAF的所在地：据报道，获得了一种全长BRAF的策略，该策略比截短的BRAF更活跃。为了更好地了解BRAF调控，对纯化的全长BRAF进行了详细的动力学分析，以研究BRAF抑制剂，MEK结合，RAS结合以及CRAF异二聚化对体外激酶活性的影响。

更新日期：2018-08-17

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