Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice,Food & Function

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Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice
Food & Function ( IF 5.1 ) Pub Date : 2018-07-11 , DOI: 10.1039/c8fo00783g
B. Medeiros-Fonseca _{1,

2,

3,

4,

5} , V. F. Mestre _{1,

2,

3,

4,

5} , B. Colaço _{1,

2,

3,

4,

6} , M. J. Pires _{1,

2,

3,

4,

5} , T. Martins _{1,

2,

3,

4,

5} , R. M. Gil da Costa _{1,

2,

3,

4,

7} , M. J. Neuparth _{4,

8,

9,

10,

11} , R. Medeiros _{4,

12,

13,

14,

15} , Magda S. S. Moutinho _{1,

2,

3,

4,

5} , Maria Inês Dias _{4,

16,

17,

18} , Lillian Barros _{4,

16,

17,

18} , M. M. S. M. Bastos _{4,

7,

15,

19,

20} , L. Félix _{1,

2,

3,

4} , C. Venâncio _{1,

2,

3,

4,

6} , Isabel C. F. R. Ferreira _{4,

16,

17,

18} , L. M. Antunes _{1,

2,

3,

4,

5} , P. A. Oliveira _{1,

2,

3,

4,

5}

Affiliation

Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB)
University of Trás-os-Montes and Alto Douro (UTAD)
Vila Real
Portugal
Department of Veterinary Sciences
Animal Science Department
Laboratory for Process Engineering Environment Biotechnology and Energy (LEPABE) Chemical Engineering Department
Advanced Polytechnic and University Cooperative (CESPU)
Institute of Research and Advanced Training in Health Sciences and Technologies (IINFACTS) Gandra
Research Center in Physical Activity
Health and Leisure (CIAFEL)
Molecular Oncology and Viral Pathology Group
IPO-Porto Research Center (CI-IPOP)
Portuguese Institute of Oncology of Porto (IPO-Porto)
Porto
Centro de Investigação de Montanha (CIMO)
Instituto Politécnico de Bragança
5300-253 Bragança
Faculty of Engineering
University of Porto (FEUP)

Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16^−/− without treatment, group II: treated HPV16^−/−, group III: HPV16^+/− without treatment and group IV: treated HPV16^+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wild-type animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.

中文翻译：

月桂（Laurus nobilis）含水叶提取物在HPV16转基因小鼠中的毒理学和抗肿瘤活性

由人乳头瘤病毒（HPV）感染引起的癌症仍然是重大的公共卫生威胁，推动了新疗法的研究。月桂树（Laurus nobilis）化合物和提取物最近显示出抗HPV转化细胞系的体外活性。这项工作旨在评估月桂树提取物在HPV16诱导的癌症的转基因小鼠模型中的体内功效和肝毒性。使用四个实验组（n = 10），连续三周在饮用水中（每只动物每天20 mg）给予提取物（组I：未经治疗的HPV16 ^-/-，组II：已治疗的HPV16 ^-/-，组III：HPV16 ^+/-未经治疗且第IV组：已治疗的HPV16 ^+/-）。在治疗期之后，处死动物并使用皮肤样品在组织学上对皮肤损伤进行分类。毒理学参数包括血液和生化血液标志物，脾和肝组织学以及肝氧化应激。在该模型中，提取物未阻止HPV16诱导的皮肤病变的进展。经处理的野生型动物显示出轻度肝炎，而转基因动物则体重减轻。但是，血液，生化和肝氧化应激指标没有变化。

更新日期：2018-08-15

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