An artificial receptor approach for proteins capture and release was designed based on ionic-sensitive and thermo-sensitive functionalized polymers, which were modified onto the surface of magnetic nanoparticles. The dual-functional poly(styrene-sulfonate-N-isopropylacrylamide) (P(SS-NIPAm)) was synthesized via the free radical polymerization method and used as the artificial receptor with electrostatic, hydrophilic and hydrophobic properties. The affinity between the artificial receptor and lysozyme was investigated using high performance liquid chromatography. Arising from the strong and specific interactions between the negatively charged sulfonic acid groups of P(SS-NIPAm) and positively charged amino groups of the protein, the prepared artificial receptor exhibited outstanding affinity for lysozyme. Interestingly, due to the thermo-responsive and ionic strength-responsive features of the prepared P(SS-NIPAm), the capture and release process for lysozyme could be modulated by varying the environmental temperature and ionic strength. Further, the proposed artificial receptor based on P(SS-NIPAm) modification of magnetic nanoparticles surface was used for affinity capture of lysozyme in human serum with a recovery ranging from 89.9% to 100.4%, paving a new way for synthesis of artificial receptors and showing great potential for recognition of target protein in real bio-samples.

中文翻译：

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用于分离溶菌酶的双功能聚合物修饰磁性纳米粒子

基于离子敏感和热敏功能化聚合物设计了一种用于蛋白质捕获和释放的人工受体方法，这些聚合物被修饰到磁性纳米粒子的表面。通过自由基聚合法合成了双功能聚（苯乙烯-磺酸盐-N-异丙基丙烯酰胺）（P（SS-NIPAm）），并将其用作具有静电、亲水和疏水特性的人工受体。人工受体和溶菌酶之间的亲和力使用高效液相色谱进行研究。由于 P(SS-NIPAm) 的带负电荷的磺酸基团与蛋白质的带正电荷的氨基之间的强烈和特异性相互作用，制备的人工受体对溶菌酶表现出出色的亲和力。有趣的是，由于所制备的 P(SS-NIPAm) 具有热响应和离子强度响应特性，可以通过改变环境温度和离子强度来调节溶菌酶的捕获和释放过程。此外，所提出的基于磁性纳米粒子表面 P(SS-NIPAm) 修饰的人工受体用于亲和捕获人血清中的溶菌酶，回收率范围为 89.9% 至 100.4%，为人工受体的合成和显示出识别真实生物样品中目标蛋白的巨大潜力。