A pulsed light emitting diode, coupled with a properly configured spectrometer is implemented in open source analytical ultracentrifugation detection hardware, allowing for spectral acquisition spanning the visible to near infrared. Details of the challenges arising with the new hardware are described, along with the necessary solutions to overcome them. The system is successfully tested with polystyrene and gold nanoparticles, demonstrating effectiveness for different material and particle geometries. Sedimentation coefficient distributions and analytically extracted spectra are presented for the multiwavelength datasets extending into the near infrared. Additionally, deconvolution algorithms are applied as a further demonstration of tools applicable to these datasets and the resolving power possible. The results of gold nanoparticle analysis presented here show effective near infrared spectral acquisition is now possible for multiwavelength analytical ultracentrifugation, opening the door for analysis of many important samples with spectral properties in this range, including rich classes of metal and semiconductor nanoparticles.

中文翻译：

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用于多波长分析超速离心的基于 LED 的近红外光谱采集：金纳米粒子的案例研究

在开源分析超速离心检测硬件中实现了一个脉冲发光二极管和一个正确配置的光谱仪，允许跨越可见光到近红外的光谱采集。描述了新硬件带来的挑战的详细信息，以及克服这些挑战的必要解决方案。该系统已通过聚苯乙烯和金纳米粒子成功测试，证明了对不同材料和粒子几何形状的有效性。提供了延伸到近红外的多波长数据集的沉降系数分布和分析提取的光谱。此外，解卷积算法被用作进一步演示适用于这些数据集的工具和可能的分辨率。