Phospholipids content in cellular and mitochondrial membranes is essential for maintaining normal function. Previous studies have found a lower polyunsaturated fatty acid (PUFA) content in mitochondria than whole tissue, theorizing decreased PUFA protects against oxidative injury. However, phospholipids (PPLs) are uniquely difficult to quantify without class separation and, as prior approaches have predominately used reverse-phase HPLC or shotgun analysis, quantitation of PPL classes may have been complicated due to the existence of numerous isobaric and isomeric species. We apply normal-phase HPLC with class separation to compare whole tissue and mitochondrial PPL profiles in rat brain, heart, kidney, and liver. In addition, we establish a novel method to ascertain PPL origin, using cardiolipin as a comparator to establish relative cardiolipin /PPL ratios. We report a higher PUFA content in tissue mitochondria driven by increased phosphatidylcholine unsaturation, suggesting mitochondria purposefully incorporate higher PUFA PPLs.

细胞膜和线粒体膜中的磷脂含量对于维持正常功能至关重要。先前的研究发现，线粒体中的多不饱和脂肪酸（PUFA）含量低于整个组织，因此从理论上讲，减少的PUFA可以防止氧化损伤。但是，如果不进行类分离，则磷脂（PPL）很难进行定量分析，而且由于现有方法已主要用于反相HPLC或shot弹枪分析，由于存在许多同量异构和同分异构体，因此PPL类的定量分析可能很复杂。我们将正相HPLC与类别分离进行比较，以比较大鼠脑，心脏，肾脏和肝脏中的整个组织和线粒体PPL谱。此外，我们建立了一种确定PPL来源的新颖方法，使用心磷脂作为比较剂来建立相对的心磷脂/ PPL比值。我们报告由增加的磷脂酰胆碱不饱和度驱动的组织线粒体中较高的PUFA含量，表明线粒体有目的地并入较高的PUFA PPL。