An assay method to quantify ondansetron (OND), granisetron (GRA) and tropisetron (TRO) in goat plasma has been successfully developed and validated. This method procedure for the analysis of OND, GRA and TRO was involved of extracting samples with hydrophilic interaction liquid chromatography (HILIC) solid phase extraction (SPE) and determination by liquid chromatography coupled to tandem mass spectroscopy. An SPE method for the simultaneous extraction of OND, GRA and TRO with high efficiency and selectivity was developed. Prior to HPLC-MS/MS analysis, most of the sources of interference present in the supernatant after protein precipitation of plasma proteins was efficiently removed from the samples by the HILIC SPE treatment. For the quantification of OND, GRA and TRO in the samples, tandem mass spectrometry operating in positive electrospray ionization mode with multiple reaction monitoring was used. The calibration curve was performed in the range of 0.2–20 ng/mL for the target OND, GRA and TRO in goat plasma samples. The precision of the intra- and inter-day assay for OND, GRA and TRO were 1.84–6.23% and 3.89–5.31%, 2.63–6.29% and 3.76–5.31%, 1.99–5.67% and 2.64–4.70%, respectively. The accuracy of the intra- and inter-day assay for OND, GRA and TRO were 89.15–97.39% and 89.46–95.17%, 91.08–100.82% and 91.24–99.47%, 92.30–100.74% and 94.21–97.90%, respectively. For the determination of OND, GRA and TRO in plasma samples, no significant matrix effects were observed. The mean absolute recoveries were 103–150%, 115–121%, and 98–141% for OND, GRA and TRO, respectively. Furthermore, the mean process efficiency values of silica SPE were 98–135%, 92–124%, and 72–109% for OND, GRA and TRO, respectively.

已经成功开发和验证了一种定量测定山羊血浆中的恩丹西酮（OND），格拉司琼（GRA）和托吡司琼（TRO）的测定方法。这种分析OND，GRA和TRO的方法包括：通过亲水相互作用液相色谱（HILIC）固相萃取（SPE）萃取样品，以及通过液相色谱-串联质谱法进行测定。建立了同时高效提取OND，GRA和TRO的SPE方法。在进行HPLC-MS / MS分析之前，血浆蛋白沉淀后，上清液中存在的大多数干扰源都可以通过HILIC SPE处理从样品中有效去除。为了定量分析样品中的OND，GRA和TRO，使用在正电喷雾电离模式下进行多重反应监测的串联质谱。山羊血浆样品中目标OND，GRA和TRO的校正曲线在0.2–20 ng / mL范围内执行。日间和日间分析OND，GRA和TRO的精度分别为1.84–6.23％和3.89–5.31％，2.63–6.29％和3.76–5.31％，1.99–5.67％和2.64–4.70％。日间和日间检测OND，GRA和TRO的准确性分别为89.15–97.39％和89.46–95.17％，91.08–100.82％和91.24–99.47％，92.30–100.74％和94.21–97.90％。在测定血浆样品中的OND，GRA和TRO时，未观察到明显的基质效应。OND，GRA和TRO的平均绝对回收率分别为103–150％，115–121％和98–141％。此外，