Building Single-Color AIE-Active Reversible Micelles to Interpret Temperature and pH Stimuli in Both Solutions and Cells,Macromolecules

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Building Single-Color AIE-Active Reversible Micelles to Interpret Temperature and pH Stimuli in Both Solutions and Cells
Macromolecules ( IF 5.1 ) Pub Date : 2018-07-09 00:00:00 , DOI: 10.1021/acs.macromol.8b00656
Yanjie Zhao ₁ , Yan Wu ₁ , Shuo Chen ₁ , Hongping Deng ₁ , Xinyuan Zhu ₁

Affiliation

Interpreting macroscopic stimulus into visible signals with nanomaterials is crucial for the development of smart nanoprobes for in situ monitoring or imaging. Building nanoprobes to translate pH and temperature stimuli into a single-color signal with a “turn-on” feature is a great challenge. Here, we aim to interpret temperature and pH stimuli into a single-color signal via aggregation-induced emission (AIE) active reversible micelles. The dual-responsive block copolymers, PNIPAM-b-P(DPA-co-TPE) and P(NIPAM-co-TPE)-b-PDPA, were conveniently prepared with similar block length but different TPE position through reversible addition–fragmentation chain-transfer (RAFT) polymerization. Both dual-responsive polymers could self-assemble into reversible micelles with AIE-active turn-on fluorescence under the stimulus of pH or temperature, respectively. The fluorescence intensity enhanced 7-fold for PNIPAM-b-P(DPA-co-TPE) and 3-fold for P(NIPAM-co-TPE)-b-PDPA by tuning the solution pH or temperature, which further showed a robust “on–off” reversibility of fluorescence signal. Through coassembly, the stimulus of either pH or temperature could be identified based on the different intensity and expansion of the emission spectra, which was further confirmed in vitro for cell imaging. Definitely, this work provides a promising approach to interpret minor pH or temperature changes in solutions and in cells.

中文翻译：

建立单色AIE活性可逆胶束以解释溶液和细胞中的温度和pH值刺激

用纳米材料将宏观刺激解释为可见信号，对于开发用于原位监测或成像的智能纳米探针至关重要。构建纳米探针以将pH和温度刺激转换为具有“开启”功能的单色信号是一个巨大的挑战。在这里，我们旨在通过聚集诱导发射（AIE）活性可逆胶束将温度和pH刺激解释为单色信号。双反应嵌段共聚物PNIPAM- b -P（DPA- co -TPE）和P（NIPAM- co -TPE）-b-PDPA可通过可逆的加成-断裂链转移（RAFT）聚合方便地制备，具有相同的嵌段长度，但在TPE位置不同。两种双响应聚合物都可以分别在pH或温度的刺激下自组装成具有AIE活性开启荧光的可逆胶束。的荧光强度增强的7倍对PNIPAM- b -P（DPA-共-TPE）和3倍为P（NIPAM-共-TPE） - b-PDPA通过调节溶液的pH或温度，进一步显示了荧光信号的强大的“开-关”可逆性。通过协同组装，可以根据发射光谱的不同强度和扩展来识别pH或温度的刺激，进一步在体外对细胞成像进行了确认。无疑，这项工作为解释溶液和细胞中较小的pH或温度变化提供了一种有前途的方法。

更新日期：2018-07-09

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