当前位置：
X-MOL 学术
›
Food Funct.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Milk fat protects Bifidobacterium animalis subsp. lactis Bb-12 from in vitro gastrointestinal stress in potentially synbiotic table spreads
Food & Function ( IF 5.1 ) Pub Date : 2018-07-09 , DOI: 10.1039/c8fo00506k Clara Simone dos Santos 1, 2, 3, 4, 5 , Cínthia Hoch Batista de Souza 1, 2, 3, 4, 5 , Marina Padilha 1, 2, 3, 4, 5 , Luiz Antonio Gioielli 1, 2, 3, 4, 5 , Juliana Neves Rodrigues Ract 1, 2, 3, 4, 5 , Susana Marta Isay Saad 1, 2, 3, 4, 5
Food & Function ( IF 5.1 ) Pub Date : 2018-07-09 , DOI: 10.1039/c8fo00506k Clara Simone dos Santos 1, 2, 3, 4, 5 , Cínthia Hoch Batista de Souza 1, 2, 3, 4, 5 , Marina Padilha 1, 2, 3, 4, 5 , Luiz Antonio Gioielli 1, 2, 3, 4, 5 , Juliana Neves Rodrigues Ract 1, 2, 3, 4, 5 , Susana Marta Isay Saad 1, 2, 3, 4, 5
Affiliation
|
The viability and the in vitro gastrointestinal survival of Bifidobacterium animalis subsp. lactis Bb-12 (Bifidobacterium Bb-12) in table spreads with different proportions of milk fat (MF) and palm olein (PO) (MF : PO 40 : 60 and MF : PO 20 : 80) were investigated for up to 28 days of storage at 5 °C. Moreover, qPCR alone and combined with propidium monoazide (PMA) were compared with the traditional plate count method for determining the in vitro gastrointestinal survival of Bifidobacterium Bb-12 in table spreads after 35 days of storage. Formulations showed probiotic viabilities ranging from 8 to 9 log CFU g−1 across the whole storage period, and the milk fat and palm olein in different concentrations did not affect this viability. Bifidobacterium Bb-12 showed good survival after six hours under in vitro simulated gastrointestinal conditions during the studied storage period, with average reductions of 1.70 (MF : PO 40 : 60) and 2.16 log CFU g−1 (MF : PO 20 : 80). The results of the qPCR with PMA treatment and the plate count method were similar and the qPCR without PMA treatment was shown to overestimate the Bifidobacterium Bb-12 populations. However, the MF : PO 40 : 60 spread showed a Bb-12 population between 0.76 and 1.43 log CFU g−1 higher than that of MF : PO 20 : 80. Thus, the results showed that table spreads, especially food matrices with a higher proportion of milk fat, are suitable for the incorporation of Bifidobacterium Bb-12.
中文翻译：
乳脂保护动物双歧杆菌亚种。潜在共生表传播中来自体外胃肠道应激的乳酸Bb-12
动物双歧杆菌亚种的生存力和体外胃肠道存活率。研究了不同比例的乳脂（MF）和棕榈油精（PO）（MF：PO 40:60和MF：PO 20:80）的餐桌酱中的乳酸菌Bb-12（双歧杆菌Bb-12）在5°C下储存。此外，将单独使用qPCR并与单叠氮化丙锭（PMA）组合的qPCR与传统平板计数法进行比较，以确定在存放35天后餐桌中双歧杆菌Bb-12的体外胃肠道存活情况。制剂显示益生菌活力范围为8到9 log CFU g -1在整个存储期间，不同浓度的乳脂和棕榈油精不会影响其生存能力。双歧杆菌Bb-12在研究的储存期间在体外模拟胃肠道条件下六个小时后表现出良好的存活率，平均减少量为1.70（MF：PO 40：60）和2.16 log CFU g -1（MF：PO 20：80） 。采用PMA处理的qPCR和平板计数法的结果相似，未进行PMA处理的qPCR显示过高估计了双歧杆菌Bb-12种群。但是，MF：PO 40:60扩散显示Bb-12种群介于0.76和1.43 log CFU g -1之间比MF：PO 20：80更高。因此，结果表明，餐桌酱，特别是乳脂比例较高的食品基质，适合掺入双歧杆菌Bb-12。
更新日期：2018-08-15
中文翻译：
乳脂保护动物双歧杆菌亚种。潜在共生表传播中来自体外胃肠道应激的乳酸Bb-12
动物双歧杆菌亚种的生存力和体外胃肠道存活率。研究了不同比例的乳脂（MF）和棕榈油精（PO）（MF：PO 40:60和MF：PO 20:80）的餐桌酱中的乳酸菌Bb-12（双歧杆菌Bb-12）在5°C下储存。此外，将单独使用qPCR并与单叠氮化丙锭（PMA）组合的qPCR与传统平板计数法进行比较，以确定在存放35天后餐桌中双歧杆菌Bb-12的体外胃肠道存活情况。制剂显示益生菌活力范围为8到9 log CFU g -1在整个存储期间，不同浓度的乳脂和棕榈油精不会影响其生存能力。双歧杆菌Bb-12在研究的储存期间在体外模拟胃肠道条件下六个小时后表现出良好的存活率，平均减少量为1.70（MF：PO 40：60）和2.16 log CFU g -1（MF：PO 20：80） 。采用PMA处理的qPCR和平板计数法的结果相似，未进行PMA处理的qPCR显示过高估计了双歧杆菌Bb-12种群。但是，MF：PO 40:60扩散显示Bb-12种群介于0.76和1.43 log CFU g -1之间比MF：PO 20：80更高。因此，结果表明，餐桌酱，特别是乳脂比例较高的食品基质，适合掺入双歧杆菌Bb-12。
京公网安备 11010802027423号