A porous silicon rugate filter (pSiRF) insulin biosensor has been constructed and systematically optimised by investigating the effects of specific experimental parameters. The pSiRF biosensor is based on an optical sensing platform using interferometric reflectance spectroscopy (IRS) to monitor insulin binding to a surface-anchored insulin binding aptamer (IBA) within the pores. The aim of this study was to determine the optimal fabrication to elicit the maximum sensor response, lowest limit of detection (LOD) and fastest response time. Thermal hydrosilylation with undecylenic acid was used as a base surface modification, with IBA attached through formation of an amide bond. The following pSiRF fabrication conditions were investigated; porous film thickness and IBA concentration in the conjugation solution. The effect of solution flow speed over the pSiRF during insulin biosensing was also investigated in an effort to improve the performance. The optimised pSiRF biosensor was successfully applied for the detection of insulin secreted from human islets on stimulation with glucose. Finally, as a proof of concept, insulin sensing in the presence of human islets on the pSiRF surface was performed to demonstrate the robust performance of the biosensor and the potential for in-situ insulin biosensing.

通过研究特定实验参数的影响，已构建了多孔皱褶硅酸盐过滤器（pSiRF）胰岛素生物传感器，并对其进行了系统优化。pSiRF生物传感器基于光学传感平台，该平台使用干涉反射光谱（IRS）来监视胰岛素与孔内表面锚定胰岛素结合适体（IBA）的结合。这项研究的目的是确定引起最大传感器响应，最低检测限（LOD）和最快响应时间的最佳制造方法。用十一碳烯酸进行热氢化硅烷化作为基础表面改性，IBA通过形成酰胺键连接。研究了以下pSiRF的制造条件；共轭溶液中的多孔膜厚度和IBA浓度。为了提高性能，还研究了胰岛素生物传感期间溶液流速对pSiRF的影响。优化的pSiRF生物传感器已成功应用于检测葡萄糖刺激下人胰岛分泌的胰岛素。最后，作为概念验证，在pSiRF表面存在人类胰岛的情况下进行了胰岛素感测，以证明该生物传感器的鲁棒性能和潜在的应用前景。原位胰岛素生物传感。