The development of efficient sensing element for rapid and sensitive measurement of glutathione is important on early detection of human diseases. Herein, we introduce the V₂O₅ nanosheets (NS) as a novel nanozyme to biomimic the oxidase activity toward the rapid detection of glutathione. The 5–6 layered V₂O₅ NS with 300–600 nm in size is successfully fabricated by ultra-sonication assisted exfoliation of bulk V₂O₅ using N, N-dimethyl formamide as the intercalation reagent. The sensing mechanism involves the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by V₂O₅ NS with the concomitant increase in absorption intensity. Upon introduction of glutathione, the oxidized TMB is converted back to its original form and results in the simultaneous decrease in peak intensity at 650 nm. The sensor exhibits a good linear response to glutathione in the range of 10–500 nM with a limit of detection of 2.4 nM. Moreover, the V₂O₅-TMB sensor exhibits a good selectivity and high specificity, which makes the detection of glutathione applicable in diluted human serum samples. Since no functionalization of the V₂O₅ NS is required, this sensing system can open a new avenue to serve as an efficiently biomimic oxidase for glutathione detection in biological fluid samples.

快速有效地检测谷胱甘肽的有效传感元件的开发对人类疾病的早期检测很重要。在这里，我们介绍V ₂ O ₅纳米片（NS）作为新型的纳米酶，以模仿氧化酶的活性，以快速检测谷胱甘肽。通过使用N，N-二甲基甲酰胺作为插层试剂，通过超声辅助剥落大体积V ₂ O ₅ ，成功制造了尺寸为300–600 nm的5–6层V ₂ O ₅ NS 。传感机制涉及V ₂ O ₅氧化3,3'，5,5'-四甲基联苯胺（TMB）NS伴随着吸收强度的增加。引入谷胱甘肽后，氧化的TMB转换回其原始形式，并导致650 nm处的峰强度同时下降。该传感器在10–500 nM的范围内对谷胱甘肽表现出良好的线性响应，检出限为2.4 nM。此外，V ₂ O ₅ -TMB传感器具有良好的选择性和高特异性，这使得谷胱甘肽的检测适用于稀释的人血清样品。由于不需要V ₂ O ₅ NS的功能化，因此该传感系统可以开辟一条新途径，以用作生物流体样品中谷胱甘肽检测的高效仿生氧化酶。