Raman Enhancement of Blood Constituent Proteins Using Graphene,ACS Photonics

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Raman Enhancement of Blood Constituent Proteins Using Graphene
ACS Photonics ( IF 6.5 ) Pub Date : 2018-07-09 00:00:00 , DOI: 10.1021/acsphotonics.8b00456
Shengxi Huang _{1,

2} , Rishikesh Pandey ₃ , Ishan Barman _{4,

5} , Jing Kong ₁ , Mildred Dresselhaus ₁

Affiliation

Raman spectroscopy has drawn considerable attention in biomedical sensing due to the promise of label-free, multiplexed, and objective analysis along with the ability to gain molecular insights into complex biological samples. However, its true potential is yet to be realized due to the intrinsically weak Raman signal. Here, we report a simple, inexpensive and reproducible signal enhancement strategy featuring graphene as a substrate. Taking key blood constituent proteins as representative examples, we show that Raman spectra acquired from biomacromolecules can be reproducibly enhanced when these molecules are placed in contact with graphene. In particular, we demonstrate that hemoglobin and albumin display significant, but different, enhancement with the enhancement factor depending on the Raman modes, excitation wavelengths, and analyte concentrations. This technique offers a new strategy for label-free biosensing owing to the molecular fingerprinting capability, signal reliability, and simplicity of the enhancement method.

中文翻译：

使用石墨烯拉曼增强血液成分蛋白

由于无标记，多重和客观分析的希望以及获得对复杂生物样品的分子洞察力的能力，拉曼光谱学已在生物医学传感领域引起了相当大的关注。然而，由于固有的弱拉曼信号，其真正的潜力尚未实现。在这里，我们报告了一种以石墨烯为底物的简单，廉价且可重现的信号增强策略。以关键的血液成分蛋白为代表的例子，我们表明，当这些分子与石墨烯接触时，从生物大分子获得的拉曼光谱可以可再现地增强。特别是，我们证明血红蛋白和白蛋白显示出显着但不同的增强，增强因子取决于拉曼模式，激发波长，和分析物浓度。由于分子指纹能力，信号可靠性和增强方法的简便性，该技术为无标记生物传感提供了新的策略。

更新日期：2018-07-09

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