Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.

中文翻译：

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全基因组C-SWAT库用于高通量酵母基因组标签

在这里，我们描述了一个用于酿酒酵母开放阅读框（ORF）的高通量标签的C-SWAT库。在5,661个菌株中，我们在每个ORF之后插入一个受体模块，该受体模块可以有效地被标签或调控元件取代。我们用靶向测序验证了文库​​，并用明亮的荧光蛋白标记了蛋白质组，以量化异源转录终止子对蛋白表达的影响并定位先前未检测到的蛋白。