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Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
Nature Methods ( IF 36.1 ) Pub Date : 2018-07-09 , DOI: 10.1038/s41592-018-0052-9
Francesca Pennacchietti 1 , Ekaterina O Serebrovskaya 2 , Aline R Faro 3 , Irina I Shemyakina 2 , Nina G Bozhanova 2 , Alexey A Kotlobay 2 , Nadya G Gurskaya 2 , Andreas Bodén 1 , Jes Dreier 1 , Dmitry M Chudakov 2 , Konstantin A Lukyanov 2 , Vladislav V Verkhusha 3 , Alexander S Mishin 2 , Ilaria Testa 1
Affiliation  

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.



中文翻译:


用于活细胞 RESOLFT 纳米镜的快速可逆光开关红色荧光蛋白



可逆光开关荧光蛋白(rsFP)作为光学纳米显微镜的标签越来越受欢迎,因为它们可以用较低的光剂量进行成像。然而,绿色 rsFP 需要紫蓝色光进行光转换,这具有潜在的光毒性和高度散射。我们开发了基于 FusionRed 的新型 rsFP,可以用绿橙光进行可逆光切换。 rsFusionReds 明亮且具有快速的光转换能力,从而能够对活细胞进行纳米级成像。

更新日期:2018-07-10
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