Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.

中文翻译：

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用于活细胞 RESOLFT 纳米镜的快速可逆光开关红色荧光蛋白

可逆光开关荧光蛋白（rsFP）作为光学纳米显微镜的标签越来越受欢迎，因为它们可以用较低的光剂量进行成像。然而，绿色 rsFP 需要紫蓝色光进行光转换，这具有潜在的光毒性和高度散射。我们开发了基于 FusionRed 的新型 rsFP，可以用绿橙光进行可逆光切换。 rsFusionReds 明亮且具有快速的光转换能力，从而能够对活细胞进行纳米级成像。