Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.

酵母库彻底改变了细胞生物学的系统研究。为了广泛增加此类文库的数量，我们使用我们之前设计的 SWAP-Tag (SWAT) 方法构建了一个包含约 5,500 株在蛋白质 N 末端携带 SWAT NOP1promoter-GFP模块的全基因组文库。此外，我们创建了六个不同的库来恢复天然调控，创建一个带有 Cherry 标签的过表达库，或者启用来自酶或荧光团的两个片段的蛋白质互补分析。我们开发了利用这些 SWAT 集合来系统地表征酵母蛋白质组的蛋白质丰度、定位、拓扑结构和相互作用的方法。