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Storage conditions modulate the metabolomic profile of a black raspberry nectar with minimal impact on bioactivity
Food & Function ( IF 5.1 ) Pub Date : 2018-07-09 00:00:00 , DOI: 10.1039/c8fo00639c Matthew D Teegarden 1 , Thomas J Knobloch , Christopher M Weghorst , Jessica L Cooperstone , Devin G Peterson
Food & Function ( IF 5.1 ) Pub Date : 2018-07-09 00:00:00 , DOI: 10.1039/c8fo00639c Matthew D Teegarden 1 , Thomas J Knobloch , Christopher M Weghorst , Jessica L Cooperstone , Devin G Peterson
Affiliation
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Pre-clinical and clinical studies suggest black raspberries (BRBs) may inhibit the development of oral cancer. Lyophilized BRB powder is commonly used in these studies, but processed BRB products are more often consumed. The objective of this work was to understand how storage conditions influence the phytochemical profile and anti-proliferative activity of a BRB nectar beverage. Untargeted UHPLC-Q-TOF-MS based metabolomics analyses demonstrated that large chemical variation was introduced by storage above −20 °C over 60 days. However, minimal change in anti-proliferative activity was observed when stored nectar extracts were applied to SCC-83-01-82 premalignant oral epithelial cells. As proof of concept, cyanidin-3-O-rutinoside and its degradation product, protocatechuic acid, were administered in different ratios maintaining an equimolar dose, and anti-proliferative activity was maintained. This study shows the utility of metabolomics to profile global chemical changes in foods, while demonstrating that isolated phytochemicals do not explain the complete bioactivity of a complex food product.
中文翻译：
储存条件可调节黑树莓花蜜的代谢组学特征，而对生物活性的影响极小
临床前和临床研究表明黑树莓（BRB）可以抑制口腔癌的发展。这些研究中通常使用冻干 BRB 粉末，但更常食用加工过的 BRB 产品。这项工作的目的是了解储存条件如何影响 BRB 花蜜饮料的植物化学特征和抗增殖活性。基于非靶向 UHPLC-Q-TOF-MS 的代谢组学分析表明，-20 °C 以上储存超过 60 天会产生巨大的化学变化。然而，当将储存的花蜜提取物应用于 SCC-83-01-82 癌前口腔上皮细胞时，观察到抗增殖活性的微小变化。作为概念证明，花青素-3- O-芸香苷及其降解产物原儿茶酸以不同比例施用，维持等摩尔剂量，并维持抗增殖活性。这项研究展示了代谢组学在描述食品中整体化学变化方面的实用性，同时证明分离的植物化学物质并不能解释复杂食品的完整生物活性。
更新日期：2018-07-09
中文翻译：
储存条件可调节黑树莓花蜜的代谢组学特征，而对生物活性的影响极小
临床前和临床研究表明黑树莓（BRB）可以抑制口腔癌的发展。这些研究中通常使用冻干 BRB 粉末，但更常食用加工过的 BRB 产品。这项工作的目的是了解储存条件如何影响 BRB 花蜜饮料的植物化学特征和抗增殖活性。基于非靶向 UHPLC-Q-TOF-MS 的代谢组学分析表明，-20 °C 以上储存超过 60 天会产生巨大的化学变化。然而，当将储存的花蜜提取物应用于 SCC-83-01-82 癌前口腔上皮细胞时，观察到抗增殖活性的微小变化。作为概念证明，花青素-3- O-芸香苷及其降解产物原儿茶酸以不同比例施用，维持等摩尔剂量，并维持抗增殖活性。这项研究展示了代谢组学在描述食品中整体化学变化方面的实用性，同时证明分离的植物化学物质并不能解释复杂食品的完整生物活性。
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