DNA Nanotweezers and Graphene Transistor Enable Label-Free Genotyping.,Advanced Materials

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DNA Nanotweezers and Graphene Transistor Enable Label-Free Genotyping.
Advanced Materials ( IF 27.4 ) Pub Date : 2018-07-09 , DOI: 10.1002/adma.201802440
Michael T Hwang ₁ , Zejun Wang ₂ , Jinglei Ping ₃ , Deependra Kumar Ban ₄ , Zi Chao Shiah ₅ , Leif Antonschmidt ₆ , Joon Lee ₁ , Yushuang Liu ₇ , Abhijith G Karkisaval ₄ , Alan T Charlie Johnson ₃ , Chunhai Fan ₂ , Gennadi Glinsky ₈ , Ratnesh Lal _{1,

4,

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Affiliation

Materials Science and Engineering Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 201800, China.
Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Department of Mechanical and Aerospace Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Department of Electrical and Computer Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.
School of Life Science, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, 010018, China.
Institute of Engineering in Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Department of Bioengineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.

Electronic DNA-biosensor with a single nucleotide resolution capability is highly desirable for personalized medicine. However, existing DNA-biosensors, especially single nucleotide polymorphism (SNP) detection systems, have poor sensitivity and specificity and lack real-time wireless data transmission. DNA-tweezers with graphene field effect transistor (FET) are used for SNP detection and data are transmitted wirelessly for analysis. Picomolar sensitivity of quantitative SNP detection is achieved by observing changes in Dirac point shift and resistance change. The use of DNA-tweezers probe with high-quality graphene FET significantly improves analytical characteristics of SNP detection by enhancing the sensitivity more than 1000-fold in comparison to previous work. The electrical signal resulting from resistance changes triggered by DNA strand-displacement and related changes in the DNA geometry is recorded and transmitted remotely to personal electronics. Practical implementation of this enabling technology will provide cheaper, faster, and portable point-of-care molecular health status monitoring and diagnostic devices.

中文翻译：

DNA 纳米镊子和石墨烯晶体管可实现无标记基因分型。

具有单核苷酸分辨率能力的电子 DNA 生物传感器对于个性化医疗来说是非常理想的。然而，现有的DNA生物传感器，特别是单核苷酸多态性（SNP）检测系统，灵敏度和特异性较差，并且缺乏实时无线数据传输。带有石墨烯场效应晶体管 (FET) 的 DNA 镊子用于 SNP 检测，数据通过无线传输进行分析。通过观察狄拉克点位移和电阻变化的变化，可以实现定量 SNP 检测的皮摩尔灵敏度。与之前的工作相比，使用具有高质量石墨烯 FET 的 DNA 镊子探针将灵敏度提高了 1000 倍以上，从而显着改善了 SNP 检测的分析特性。由 DNA 链位移和 DNA 几何相关变化触发的电阻变化产生的电信号被记录并远程传输到个人电子设备。这种支持技术的实际实施将提供更便宜、更快、便携式的护理点分子健康状态监测和诊断设备。

更新日期：2018-07-09

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