A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging,Angewandte Chemie International Edition

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A Highly Photostable Near‐Infrared Labeling Agent Based on a Phospha‐rhodamine for Long‐Term and Deep Imaging
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-07-16 , DOI: 10.1002/anie.201804731
Marek Grzybowski ₁ , Masayasu Taki _{1,

2} , Kieko Senda ₃ , Yoshikatsu Sato ₁ , Tetsuro Ariyoshi ₄ , Yasushi Okada _{4,

5} , Ryosuke Kawakami ₆ , Takeshi Imamura ₆ , Shigehiro Yamaguchi _{1,

3}

Affiliation

Various fluorescence microscopy techniques require bright NIR‐emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus‐substituted rhodamine dyes (PORs) upon substitution at the 9‐position with a 2,6‐dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long‐term and three‐color imaging in the vis‐NIR range. Moreover, the high fluorescence longevity of PREX 710 allows tracking a dye‐labeled biomolecule by single‐molecule microscopy under physiological conditions. Deep imaging of blood vessels in mice brain has also been achieved using the bright NIR‐emitting PREX 710‐dextran conjugate.

中文翻译：

基于磷脂酰罗丹明的高光稳定性近红外标记剂，可用于长期和深层成像

各种荧光显微镜技术要求具有高化学和光稳定性的明亮的近红外发射荧光团。现在，据报道，在9位被2,6-二甲氧基苯基取代后，磷取代的罗丹明染料（POR）的性能得到了显着改善。如此获得的染料PREX 710用于染色活细胞中的线粒体，从而可以在vis-NIR范围内进行长期和三色成像。此外，PREX 710的高荧光寿命允许在生理条件下通过单分子显微镜跟踪染料标记的生物分子。使用明亮的近红外发射PREX 710-右旋糖酐共轭物，还可以对小鼠大脑的血管进行深层成像。

更新日期：2018-07-16

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