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Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-07-07 00:00:00 , DOI: 10.1039/c8lc00486b Alexandre Chícharo 1, 2, 3, 4, 5 , Marco Martins 1, 2, 3 , Lester C. Barnsley 6, 7, 8, 9, 10 , Amal Taouallah 1, 2, 3 , João Fernandes 1, 2, 3 , Bruno F. B. Silva 1, 2, 3 , Susana Cardoso 3, 11, 12 , Lorena Diéguez 1, 2, 3 , Begoña Espiña 1, 2, 3 , Paulo P. Freitas 1, 2, 3, 4, 5
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-07-07 00:00:00 , DOI: 10.1039/c8lc00486b Alexandre Chícharo 1, 2, 3, 4, 5 , Marco Martins 1, 2, 3 , Lester C. Barnsley 6, 7, 8, 9, 10 , Amal Taouallah 1, 2, 3 , João Fernandes 1, 2, 3 , Bruno F. B. Silva 1, 2, 3 , Susana Cardoso 3, 11, 12 , Lorena Diéguez 1, 2, 3 , Begoña Espiña 1, 2, 3 , Paulo P. Freitas 1, 2, 3, 4, 5
Affiliation
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We report the design and characterization of a lateral and vertical hydrodynamic focusing feature for whole cell detection on a miniaturized flow cytometer. The developed system, based on magnetic sensing, incorporates spin valve sensors on the bottom of the microfluidic channels that detect cells labeled with magnetic beads. An adaptable 3D hydrodynamic focusing system was developed that pushes labeled cells towards the bottom of the microchannel, closer to the sensors, allowing increased signal amplitude for cells labeled with magnetic beads and enhanced discrimination of labeled cells. Fluorescence microscopy indicates that the lateral and vertical hydrodynamic focusing effect was adequately implemented, consistent with simulation predictions. The sensitivity of the system to detect labeled cells was improved by at least two-fold. By estimating the coverage of magnetic beads on cells, the signal from labeled cells could be predicted using a mathematical model, which also demonstrated the sensitivity of the signal to the height of the cells relative to the sensor. The system is versatile allowing interchangeable flow rates for cells with different diameters.
中文翻译：
具有3D流动聚焦功能的增强型磁性微细胞计数仪可进行细胞计数†
我们报告了一个小型流式细胞仪上用于全细胞检测的横向和垂直流体动力学聚焦特征的设计和表征。基于磁感应的已开发系统在微流体通道底部结合了旋转阀传感器，可检测标记有磁珠的细胞。开发了一种可适应的3D流体动力聚焦系统，该系统将标记的细胞推向微通道的底部，更靠近传感器，从而使用磁珠标记的细胞的信号幅度增加，并增强了标记的细胞的辨别力。荧光显微镜表明，横向和垂直流体动力聚焦效果得到了充分的实现，与模拟预测一致。该系统检测标记细胞的灵敏度提高了至少两倍。通过估计细胞上磁珠的覆盖范围，可以使用数学模型预测来自标记细胞的信号，该数学模型还证明了信号对细胞相对于传感器高度的敏感性。该系统用途广泛，可为不同直径的细胞提供可互换的流速。
更新日期：2018-07-07
中文翻译：
具有3D流动聚焦功能的增强型磁性微细胞计数仪可进行细胞计数†
我们报告了一个小型流式细胞仪上用于全细胞检测的横向和垂直流体动力学聚焦特征的设计和表征。基于磁感应的已开发系统在微流体通道底部结合了旋转阀传感器，可检测标记有磁珠的细胞。开发了一种可适应的3D流体动力聚焦系统，该系统将标记的细胞推向微通道的底部，更靠近传感器，从而使用磁珠标记的细胞的信号幅度增加，并增强了标记的细胞的辨别力。荧光显微镜表明，横向和垂直流体动力聚焦效果得到了充分的实现，与模拟预测一致。该系统检测标记细胞的灵敏度提高了至少两倍。通过估计细胞上磁珠的覆盖范围，可以使用数学模型预测来自标记细胞的信号，该数学模型还证明了信号对细胞相对于传感器高度的敏感性。该系统用途广泛，可为不同直径的细胞提供可互换的流速。
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