The utilization of the juice from the cheapest carbon source oil palm frond (22 $/ton) in a 3.5 L bioreactor was proven to be competent in replacing pure sugar in succinic acid production. The current study provides an in-depth discussion on the utilization of CO₂ in the production of succinic acid. In the serum-bottle fermentation, the yield of succinic acid increased by 51% from 0.47 to 0.71 g/g while the final titer of succinic acid enhanced by 4 fold were attributed to the anaplerotic reaction of Actinobacillus succinogenes and the alkalinity effect of the dissolved CO₂. In the bioreactor, the highest mass transfer in the fermentation medium was achieved at 0.5 vvm CO₂ at which 3 M KOH was pumped in at a rate of 2.0 mL/min to neutralize the carbonic acid formation, which afforded the highest succinic acid concentration of 30.7 g/L. A first report on actual stoichiometry equation relating substrate, CO₂ consumption and all products formation was derived from the results of bioreactor fermentation to lay as a guideline for future reference of actual succinic acid production reaction. C₆H₁₂O₆ + 0.145 NH₃ + 0.642 CO₂ → 1.083 C₄H₆O₄ + 0.509 CH₂O₂ + 0.420 C₂H₄O₂ + 0.117 C₂H₅OH + 0.726 CH_1.8N_0.2O_0.5 + 0.614 H₂O. Actual stoichiometry equation revealed that for every kg of succinic acid produced, 0.22 kg of CO₂ will be absorbed. The desirable effect of CO₂ on the production of succinic acid may translate into a mutually-beneficial relationship between the succinic acid and the CO₂-generating biofuel industries.

事实证明，在3.5升生物反应器中利用最便宜的碳源油棕叶（22美元/吨）榨汁可替代琥珀酸生产中的纯糖。本研究对琥珀酸生产中CO ₂的利用进行了深入的讨论。在血清瓶发酵中，琥珀酸的产率从0.47增至0.71 g / g，增加了51％，而最终的琥珀酸滴度提高了4倍，这归因于放线杆菌的琥珀酸放线菌反应和碱度的影响。 CO ₂。在生物反应器中，在0.5 vvm CO ₂的发酵培养基中实现了最高的传质然后以2.0 mL / min的速度泵入3 M KOH以中和碳酸的生成，从而提供最高的琥珀酸浓度30.7 g / L。有关底物，CO ₂消耗量和所有产物形成的实际化学计量方程的第一份报告是根据生物反应器发酵的结果得出的，为将来实际生产琥珀酸的反应提供了参考。C ₆ H ₁₂ O ₆  + 0.145 NH ₃  + 0.642 CO ₂  →1.083 C ₄ H ₆ O ₄  + 0.509 CH ₂ O ₂  + 0.420 C ₂ H ₄ O₂  + 0.117 c ^ ₂ ^ h ₅ OH + 0.726 CH _1.8 Ñ _0.2 ø _0.5  + 0.614ħ ₂ O.实际化学计量方程表明，对于每千克产生的琥珀酸，0.22千克的CO ₂将被吸收。CO ₂对琥珀酸生产的理想作用可以转化为琥珀酸与产生CO _2的生物燃料工业之间的互利关系。