Abstract Receptor for advanced glycation end-products (RAGE) is overexpressed in various cancer cells. In this study, a RAGE-binding peptide (RBP) was conjugated to polyethylenimine (25 kDa, PEI). RBP-conjugated PEI (PEI-RBP) was characterized as a dual-functional reagent, a RAGE-mediated gene carrier and an anti-angiogenic reagent. As a gene carrier, PEI-RBP had higher transfection efficiency to the C6 glioblastoma cells than PEI. As an anti-angiogenic reagent, the pEmpty/PEI-RBP complex reduced RAGE expression on the surface of the C6 glioblastoma cells. Also, the complex reduced the VEGF expression and tube formation of endothelial cells. Therefore, PEI-RBP may be useful for development of glioblastoma therapy.

中文翻译：

---

作为双功能载体的 RAGE 结合肽偶联聚乙烯亚胺：RAGE 介导的基因载体和抗血管生成试剂

摘要 晚期糖基化终产物 (RAGE) 受体在多种癌细胞中过度表达。在本研究中，RAGE 结合肽 (RBP) 与聚乙烯亚胺 (25 kDa，PEI) 偶联。RBP 偶联的 PEI (PEI-RBP) 被表征为双功能试剂、RAGE 介导的基因载体和抗血管生成试剂。作为基因载体，PEI-RBP对C6胶质母细胞瘤细胞的转染效率高于PEI。作为一种抗血管生成试剂，pEmpty/PEI-RBP 复合物降低了 C6 胶质母细胞瘤细胞表面的 RAGE 表达。此外，该复合物降低了内皮细胞的 VEGF 表达和管形成。因此，PEI-RBP 可能有助于胶质母细胞瘤治疗的发展。