This study aims to establish the biological and chemical profile of Asphodeline liburnica (Scop.) Rchb. root. The antioxidant, antimicrobial, enzyme inhibitory, DNA protection, apoptotic DNA ladder fragmentation analysis, and anti-proliferative of A. liburnica were established using standard assays. In silico study was also performed to understand interactions between quantified anthraquinones and key enzymes of clinical relevance. Total phenolic and flavonoid contents were found to be 9.67 mgGAE/g and 1.48 mgRE/g extract, respectively. Chrysophanol was detected as a major anthraquinone. The extract exhibited radical scavenging ability against DPPH and ABTS with values of 13.23 and 66.99 mgTE/g extract, respectively. Good inhibitory activity against tyrosinase was recorded. In silico experiments showed that the anthraquinones were able to establish coordinative bonds with the copper atoms present in the enzymatic cavity of tyrosinase. MTT cell viability test on MDA-MB-231 cells showed that at 0.1 and 1 μg of extracts induced anti-proliferative effect. Apoptotic DNA fragmentation analysis indicated nuclear condensation resulting in DNA fragmentation, which exhibited apoptotic cell death in the presence of A. liburnica. This study has provided insights on the potential usage of A. liburnica which could open new avenues for research and stimulate future interest for the development of safe novel biopharmaceuticals.

这项研究的目的是建立天竺葵（Spho。）Rchb的生物学和化学特性。根。使用标准测定方法建立了天竺葵的抗氧化剂，抗菌剂，酶抑制剂，DNA保护剂，凋亡性DNA梯形片段分析法和抗增殖剂。在计算机上还进行了一项研究，以了解量化的蒽醌与临床相关的关键酶之间的相互作用。发现总的酚类和类黄酮含量分别为9.67 mgGAE / g和1.48 mgRE / g提取物。邻苯三酚被认为是一种主要的蒽醌。提取物表现出对DPPH和ABTS的自由基清除能力，分别为13.23和66.99 mgTE / g提取物。记录了对酪氨酸酶的良好抑制活性。在计算机上实验表明，蒽醌能够与酪氨酸酶酶腔中存在的铜原子建立配位键。在MDA-MB-231细胞上进行的MTT细胞活力测试表明，在0.1和1μg的提取物中诱导了抗增殖作用。凋亡的DNA片段分析表明核浓缩导致DNA片段化，在有荚。曲霉存在的情况下，凋亡细胞死亡。这项研究提供了关于潜在的使用A. liburnica的见解，这可以为研究开辟新的途径，并激发人们对开发安全的新型生物药物的兴趣。