Transferrin (TrF) is a very important human body glycoprotein and a clinical biomarker which controls the body’s iron ion channels and iron ion balance. Any change in TrF concentration and isoform also reflects the emergence of some diseases. In this work, we prepared magnetic molecularly imprinted nanoparticles (deep eutectic solvent-molecular imprinting polymers [DES-MIPs]) with a deep eutectic solvent (DES) as a functional monomer to separate TrF in human serum. The DES dosage for MIP, pH value, and time for adsorption have been optimized, and these materials show special adsorption properties for TrF. The maximum adsorption capacity (Q_max) and dissociation constant K_L of the MIP by the Langmuir adsorption curve (R² = 0.9949) were 37.5 mg/g and 0.015 g/L, respectively. The imprinting factor of the MIP is 3.50 with relative standard deviation (5.63%). In summary, the use of DES as a functional monomer in molecular imprinting technology provides a novel, efficient, and biocompatible method for the isolation and purification of proteins.

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转铁蛋白（TrF）是一种非常重要的人体糖蛋白，是控制人体铁离子通道和铁离子平衡的临床生物标志物。TrF浓度和同工型的任何变化也反映出某些疾病的出现。在这项工作中，我们制备了磁性分子印迹纳米粒子（深共熔溶剂-分子印迹聚合物[DES-MIPs]），其中深共熔溶剂（DES）作为功能单体分离人血清中的TrF。优化了DES的MIP用量，pH值和吸附时间，这些材料对TrF具有特殊的吸附性能。由Langmuir吸附曲线（R ^2）得出的MIP的最大吸附量（Q _max）和离解常数K _L。 = 0.9949）分别为37.5 mg / g和0.015 g / L。MIP的压印因子为3.50，相对标准偏差（5.63％）。总之，在分子印迹技术中使用DES作为功能性单体为分离和纯化蛋白质提供了一种新颖，有效且生物相容的方法。

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