Background

Continuous transformation and development of new detection tools for bacteria has converted the laborious scientific work into smart apparatus in recent years. The journey had begun with the culture-based plate enumeration, and now it has evolved into several culture-independent techniques. Polymerase chain reaction (PCR) is on the top of the list that is now a routinely used biological approach to detect bacterial cells. Instrumental techniques are also helpful in this regard, as they are more sensitive for detection of various microbes.

Scope and approach

In this review, we described new trends and their practical application in the fields of detection microbiology and food technology. This study provides a brief overview of conventional and modern detection techniques which includes nucleic-acid sequence based techniques to non-destructive imaging techniques.

Key findings and conclusions

Besides the availability of antibiotics and clinical treatments, bacterial infections significantly increase the mortality rate. It is necessary to detect apparent infectious agents beforehand. Therefore, the detection methods for microorganisms should be more rapid, smart and reliable in response to the need. Conventional detection techniques are slow and time-consuming but more accurate and reliable than the modern detection techniques. By combing the mentioned techniques, scientists can achieve better results.

中文翻译：

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食品科学中病原细菌的常规和新兴检测技术：综述

背景

近年来，新的细菌检测工具的不断改造和开发已将艰巨的科研工作转变为智能设备。此旅程始于基于文化的板块枚举，现在已演变为多种与文化无关的技术。聚合酶链反应（PCR）在列表的顶部，现在已成为检测细菌细胞的常规生物学方法。仪器技术在这方面也很有帮助，因为它们对于检测各种微生物更加敏感。

范围和方法

在这篇综述中，我们描述了检测微生物学和食品技术领域中的新趋势及其实际应用。这项研究简要概述了常规和现代检测技术，包括基于核酸序列的技术到无损成像技术。

主要发现和结论

除了提供抗生素和临床治疗外，细菌感染还大大增加了死亡率。有必要事先检测出明显的传染原。因此，根据需要，微生物的检测方法应该更加快速，智能和可靠。常规检测技术速度慢，费时，但比现代检测技术更准确，更可靠。通过结合上述技术，科学家可以取得更好的结果。