Laccases are capable of rapidly oxidizing benzo[a]pyrene. It is thought that the metabolites with an increase in water solubility caused by the oxidation of benzo[a]pyrene may stimulate the subsequent mineralization. However, to date, there has been no experimental evidence to support this. In this study, the fate of benzo[a]pyrene in soil affected by laccase amendment and the resulting soil bacterial responses were investigated. Laccase amendment promoted benzo[a]pyrene dissipation (15.6%) from soil, accompanied by trace mineralization (<0.58 ± 0.02%) and substantial bound residue formation (∼80%). An increase of ∼15% in the bound residue fraction was observed by laccase amendment, which mainly resulted from covalent binding of the residues to humin fraction. During the incubation, the abundance of bacterial 16S rRNA and polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase genes did not change markedly. In contrast, benzo[a]pyrene treated with laccase resulted in a smaller shift in the bacterial community composition, indicating a reduced disturbance to the soil microbial communities. These results here suggest that benzo[a]pyrene contaminated soil can be detoxified by laccase amendment mainly due to the enhanced bound residue formation to soil organic matter via covalent binding.

漆酶能够快速氧化苯并[ a ] py。据认为，由苯并[ a ] py的氧化引起的水溶性增加的代谢物可能会刺激随后的矿化。但是，迄今为止，还没有实验证据支持这一点。在这项研究中，调查了受漆酶修饰影响的土壤中苯并[ a ] re的去向以及由此产生的土壤细菌反应。漆酶修饰促进了苯并[ a]py从土壤中的消散（15.6％），伴有微量矿化（＜0.58±0.02％）和大量结合残留物形成（约80％）。通过漆酶修饰观察到结合的残基部分增加了约15％，这主要是由于残基与腐殖质部分的共价结合所致。在孵育过程中，细菌16S rRNA和多环芳烃环羟化双加氧酶基因的丰度没有明显变化。相反，用漆酶处理的苯并[ a ] re导致细菌群落组成的变化较小，表明对土壤微生物群落的干扰减少。这些结果表明，苯并[ apy污染的土壤可以通过漆酶修饰来排毒，这主要是由于通过共价结合增加了残留物与土壤有机物的结合形成。