In this review, an assessment of non-separative methods based on mass spectrometry used to analyse volatile organic compounds in the field of bioanalysis is performed. The use of non-separative methods based on mass spectrometry has been established as an attractive option for analysing compounds. These instrumental configurations are suitable for biomedical applications because of their versatility, rapid output of results, and the wide range of volatile organic compounds that can be determined. Here, techniques such as headspace sampling coupled to mass spectrometry, membrane introduction mass spectrometry, selected ion flow tube mass spectrometry, proton transfer reaction mass spectrometry, secondary electrospray ionization mass spectrometry and ion mobility mass spectrometry, are evaluated. Samples involving non-invasive methods of collection, such as urine, saliva, breath and sweat, are mainly considered. To the best of our knowledge, a comprehensive review of all the non-separative instrumental configurations applied to the analysis of gaseous samples from all matrices non-invasively collected has not yet been carried out. The assessment of non-separative techniques for the analysis of these type of samples can be considered a key issue for future clinical applications, as they allow real-time sample analysis, without patient suffering. Any contribution to the early diagnosis of disease can be considered a priority for the scientific community. Therefore, the identification and determination of volatile organic compounds related to particular diseases has become an important field or research.

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基于挥发性有机化合物的非侵入性医学诊断的非分离质谱方法：综述

在这篇综述中，对用于分析生物分析领域挥发性有机化合物的基于质谱的非分离方法进行了评估。使用基于质谱的非分离方法已被确立为分析化合物的有吸引力的选择。这些仪器配置适用于生物医学应用，因为它们具有多功能性、快速的结果输出以及可以测定的范围广泛的挥发性有机化合物。在这里，评估了诸如顶空采样与质谱联用、膜引入质谱、选择离子流管质谱、质子转移反应质谱、二次电喷雾电离质谱和离子迁移质谱等技术。涉及非侵入性收集方法的样本，如尿液、唾液、呼吸和汗水，主要考虑。据我们所知，尚未对所有非侵入式收集的所有基质中的气体样品进行分析的所有非分离仪器配置进行全面审查。用于分析这些类型样品的非分离技术的评估可被视为未来临床应用的关键问题，因为它们允许实时样品分析，而无需患者痛苦。对疾病早期诊断的任何贡献都可以被视为科学界的优先事项。因此，与特定疾病相关的挥发性有机化合物的鉴定和测定已成为一个重要的领域或研究。尚未对所有非侵入式收集的所有基质中的气体样品进行分析的所有非分离仪器配置进行全面审查。用于分析这些类型样品的非分离技术的评估可被视为未来临床应用的关键问题，因为它们允许实时样品分析，而无需患者痛苦。对疾病早期诊断的任何贡献都可以被视为科学界的优先事项。因此，与特定疾病相关的挥发性有机化合物的鉴定和测定已成为一个重要的领域或研究。尚未对所有非侵入式收集的所有基质中的气体样品进行分析的所有非分离仪器配置进行全面审查。用于分析这些类型样品的非分离技术的评估可被视为未来临床应用的关键问题，因为它们允许实时样品分析，而无需患者痛苦。对疾病早期诊断的任何贡献都可以被视为科学界的优先事项。因此，与特定疾病相关的挥发性有机化合物的鉴定和测定已成为一个重要的领域或研究。