New dual electrochemical and fluorescence sensitive curcumin-graphene quantum dots sensing platform coated on the transparent Indium-Tin-Oxide electrode was developed to sense APOe4 DNA, responsible of Alzheimer's disease. Curcumin molecule with its dual fluorescence and electrochemical properties was electropolymerized on GQDs-ITO surface. EDC/NHS chemistry was used to covalently immobilize an amino-substituted DNA probe via a malonic acid spacer. Quenching of curcumin signals following hybridized DNA complex was employed to quantify APOe4 DNA. Amperometric studies revealed an ultrasensitive behavior toward the formation of DNA complex with a sensitivity of 4.74 nA.mL.pg -1 and a limit of detection as low as 0.48 pg mL -1. The platform exhibits very good performances such as repeatability, reproducibility, selectivity and long storage stability. Fluorescence results were established for the support and the complementarity of electrochemical results. Founded results confirmed the ultrasensitivity of platform with comparable performances. Recorded results in human blood plasma demonstrated the high efficacy of curcumin system sensing even in the clinical matrix.

中文翻译：

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姜黄素-石墨烯量子点用于双模传感平台：APO e4 的电化学和荧光检测，负责阿尔茨海默病

开发了涂覆在透明氧化铟锡电极上的新型双电化学和荧光敏感姜黄素-石墨烯量子点传感平台，用于检测导致阿尔茨海默病的 APOe4 DNA。在 GQDs-ITO 表面电聚合具有双重荧光和电化学性质的姜黄素分子。EDC/NHS 化学用于通过丙二酸间隔物共价固定氨基取代的 DNA 探针。使用杂交 DNA 复合物后姜黄素信号的淬灭来量化 APOe4 DNA。电流分析研究揭示了对 DNA 复合物形成的超灵敏行为，灵敏度为 4.74 nA.mL.pg -1，检测限低至 0.48 pg mL -1。该平台表现出非常好的性能，如重复性、再现性、选择性和长期储存稳定性。荧光结果建立了电化学结果的支持和互补性。建立的结果证实了具有可比性能的平台的超灵敏性。人体血浆中的记录结果证明了姜黄素系统即使在临床基质中也具有高效感。