A sensitive LC–MS/MS method was established to quantify diols and glycerol in fermentation broth using trichloroacetyl isocyanate as instantaneous derivatization reagent for monitoring the production of 1,3-propanediol and 2,3-butanediol from the biodiesel biorefinery process. Due to the derivatization reaction was very quickly at room temperature, only 1 min was needed for the reaction process. In addition, both extraction of analytes and evaporation of water were not employed in the analytical procedure. Furthermore, the isotope of chlorine was beneficial for understanding of the secondary mass spectrum and avoiding false positive results. Therefore, much more accurate results of diols and glycerol concentration in fermentation could be obtained even at very low levels for the evaluation of microbial metabolism pathway modification.

建立了灵敏的LC-MS / MS方法，使用三氯乙酰基异氰酸酯作为瞬时衍生试剂来定量发酵液中的二醇和甘油，以监测生物柴油生物提炼过程中1,3-丙二醇和2,3-丁二醇的生产。由于在室温下衍生化反应非常快，因此反应过程仅需要1分钟。另外，在分析过程中既没有提取分析物，也没有蒸发水。此外，氯的同位素对于理解次级质谱图和避免假阳性结果是有益的。因此，即使在非常低的水平下，对于微生物代谢途径修饰的评估，也可以获得更准确的发酵中二醇和甘油浓度的结果。