A total of 48 chlorophylls and derivatives were identified and successfully determined in tea and processed vegetable and fruit foodstuff by UHPLC with photodiode-array and mass spectrometry detection. The method allowed the proper separation of chlorophyll derivatives resulting from demetallation, dephytilation, decarbomethoxylation, epimerisation and copperisation. The method was performed in less than 12 min, using an optimised ternary gradient (MeOH, iPrOH, MeCN and H₂O with 10 mM of ammonium acetate) on an ACQUITY HSS T3 column. Mass spectrometry, applying both ESI and APCI ionization sources, was used for identification purposes. The method was applied to evaluate the degree of processing in teas of different origin and quality. It allowed differentiation between supermarket own-brand tea bags and teas sold by specialised shops. Pheophytins, pheophorbides and pyro derivatives were found mainly in processed green vegetable and fruit products thereof. However, chlorophyll-derived food colorants, such as Cu-chlorophyllins, Cu-pheophytins, Cu-pyropheophytins, Cu-pheophorbides and Cu-pyropheophorbides, were also detected in several products.

借助光电二极管阵列和质谱检测的UHPLC，共鉴定并成功测定了茶叶，蔬菜和水果加工食品中的48种叶绿素及其衍生物。该方法允许适当分离由脱金属，脱金属，脱甲氧甲氧基化，差向异构化和铜化产生的叶绿素衍生物。使用优化的三元梯度（MeOH，iPrOH，MeCN和H ₂O在ACQUITY HSS T3色谱柱上含10 mM乙酸铵）。使用ESI和APCI电离源的质谱用于鉴定目的。该方法用于评估不同来源和品质的茶的加工程度。它允许区分超市自有品牌的茶袋和专门商店出售的茶。卟啉，脱镁叶绿酸和焦磷酸衍生物主要存在于经过加工的绿色蔬菜和水果产品中。但是，在几种产品中也检测到了叶绿素衍生的食品着色剂，例如铜叶绿素，铜脱镁叶绿素，铜焦脱镁叶绿素，铜脱镁叶绿素和铜脱镁叶绿素。