AbstractA method was developed for the determination of mercuric ion Hg(II). It is based on hybridization chain reaction (HCR) and surface-enhanced Raman scattering (SERS). Raman signal DNA and streptavidin were self-assembled on gold nanoparticles as a novel signal nanoprobe (AuNP-sDNA). A thymine-mercury(II)-thymine structure was immobilized on magnetic beads (MBs). The HCR makes use of two hairpin probes that are initiated by the trigger DNA to form a stable nicked dsDNA structure (MB-TS-hDNAs). A large number of the binding sites is provided to connect the signal nanoprobe. The stable sandwich structure (MB-TS-hDNA/AuNP-sDNA) was isolated by applying a magnetic field and used in the amplification step. In this way, Hg(II) can be determined sensitively after multiple signal amplification. The SERS signal, measured at 1499 cm−1, increases linearly in the 0.1 pM to 10 nM Hg(II) concentration range, and the limit of detection is 0.08 pM (at an S/N ratio of 3). The method was applied to the detection of Hg(II) in spiked environment water samples, with recoveries ranging from 96 to 119%. Graphical abstractSchematic of a method based on the use of a stable T-Hg(II)-T structure and a self-assembled nanoprobe. It was applied to the trace Hg(II) detection based on hybridization chain reaction (HCR) and surface-enhanced Raman scattering (SERS).

中文翻译：

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基于杂交链循环放大反应和金纳米粒子表面增强拉曼散射的 Hg(II) 灵敏测定

摘要 建立了测定汞离子Hg(II)的方法。它基于杂交链反应 (HCR) 和表面增强拉曼散射 (SERS)。拉曼信号 DNA 和链霉亲和素自组装在金纳米粒子上作为新型信号纳米探针 (AuNP-sDNA)。胸腺嘧啶-汞 (II)-胸腺嘧啶结构固定在磁珠 (MB) 上。HCR 利用由触发 DNA 启动的两个发夹探针形成稳定的带切口 dsDNA 结构 (MB-TS-hDNA)。提供了大量的结合位点来连接信号纳米探针。通过施加磁场分离稳定的夹心结构 (MB-TS-hDNA/AuNP-sDNA) 并用于扩增步骤。这样，经过多次信号放大后，可以灵敏地测定 Hg(II)。在 1499 cm−1 处测量的 SERS 信号，在 0.1 pM 至 10 nM Hg(II) 浓度范围内线性增加，检测限为 0.08 pM（信噪比为 3）。该方法用于检测加标环境水样中的 Hg(II)，回收率范围为 96% 至 119%。基于使用稳定的 T-Hg(II)-T 结构和自组装纳米探针的方法的图形摘要示意图。应用于基于杂交链反应（HCR）和表面增强拉曼散射（SERS）的痕量汞（II）检测。基于使用稳定的 T-Hg(II)-T 结构和自组装纳米探针的方法的图形摘要示意图。应用于基于杂交链反应（HCR）和表面增强拉曼散射（SERS）的痕量汞（II）检测。基于使用稳定的 T-Hg(II)-T 结构和自组装纳米探针的方法的图形摘要示意图。应用于基于杂交链反应（HCR）和表面增强拉曼散射（SERS）的痕量汞（II）检测。