AbstractThis study reports on a method for fluorometric aptasensing of adenosine triphosphate (ATP). It is based on the interaction of dispersed (red) and agglomerated (blue) gold nanoparticles (AuNPs) with a water-dispered terbium(III) based metal-organic framework (Tb-MOF). The dispersed AuNPs quench the emissions of the Tb-MOF, while the aggregated AuNPs have little effect. Under the condition of high salt concentration, the free aptamer against ATP does not stabilize the AuNPs against aggregation. This causes a color change from red to blue and weak quenching of the fluorescence of the Tb-MOF (with peaks at 489 nm and 544 nm after excitation at 290 nm). On addition of ATP, it will be bound by its aptamer to form a complex that is adsorbed on the AuNPs. This protects the AuNPs from salt-induced aggregation and the color (with a peak at 525 nm) remains red. The two fluorescence bands of the Tb-MOF are therefore suppressed by fluorescence resonance energy transfer (FRET) between Tb-MOF and the dispersed AuNPs. Fluorescence drops linearly in the 50 nM to 10 μM ATP concentration range, and the detection limit is 23 nM. ATP analogs such as guanosine triphosphate, uridine triphosphate, cytidine triphosphate, adenosine monophosphate and cyclic adenosine monophosphate have no obvious interference. The method was successfully applied to the determination of ATP in (spiked) human plasma samples and gave satisfactory recoveries. Graphical abstractSchematic of a terbium-based metal-organic framework@gold nanoparticle system as a fluorometric probe for aptamer based determination of adenosine triphosphate. The dispersed gold nanoparticles (AuNPs) quench the fluorescence of the terbium-based metal-organic framework (Tb-MOF), while the aggregated AuNPs have little effect. In the presence of adenosine triphosphate (ATP), the aptamer-ATP complexes provide greater protection towards AuNPs than aptamer alone under high salt condition. Based on this, a novel Tb-MOF@AuNP platform is established for ATP detection.

中文翻译：

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铽基金属有机骨架@金纳米颗粒系统作为荧光探针用于基于适配体测定三磷酸腺苷

摘要本研究报告了一种三磷酸腺苷 (ATP) 荧光适配传感的方法。它基于分散的（红色）和团聚的（蓝色）金纳米粒子 (AuNPs) 与水分散的铽 (III) 基金属有机框架 (Tb-MOF) 的相互作用。分散的 AuNPs 抑制了 Tb-MOF 的发射，而聚集的 AuNPs 几乎没有影响。在高盐浓度条件下，抗 ATP 的游离适体不能稳定 AuNPs 的聚集。这会导致颜色从红色变为蓝色，并且 Tb-MOF 的荧光减弱（在 290 nm 激发后在 489 nm 和 544 nm 处出现峰值）。添加 ATP 后，它将与其适体结合，形成吸附在 AuNP 上的复合物。这可以保护 AuNP 免受盐诱导的聚集，并且颜色（峰值在 525 nm）保持红色。因此，Tb-MOF 和分散的 AuNP 之间的荧光共振能量转移 (FRET) 抑制了 Tb-MOF 的两个荧光带。荧光在 50 nM 至 10 μM ATP 浓度范围内线性下降，检测限为 23 nM。三磷酸鸟苷、三磷酸尿苷、三磷酸胞苷、一磷酸腺苷、环磷酸腺苷等ATP类似物无明显干扰。该方法已成功应用于（加标）人血浆样品中 ATP 的测定，并获得了令人满意的回收率。图形摘要基于铽的金属有机框架@金纳米粒子系统作为荧光探针的示意图，用于基于适体测定三磷酸腺苷。分散的金纳米粒子 (AuNPs) 淬灭了铽基金属有机骨架 (Tb-MOF) 的荧光，而聚集的 AuNPs 几乎没有影响。在三磷酸腺苷 (ATP) 的存在下，适体-ATP 复合物在高盐条件下比单独的适体对 AuNP 提供更大的保护。在此基础上，建立了一种用于ATP检测的新型Tb-MOF@AuNP平台。