The objective of this work was on the one hand to assess the antibacterial activity of amines anchored to the external surface of mesoporous silica particles against Listeria monocytogenes in comparison with the same dose of free amines as well. It was also our aim to elucidate the mechanism of action of the new antimicrobial device. The suitability of silica nanoparticles to anchor, concentrate and improve the antimicrobial power of polyamines against L. monocytogenes has been demonstrated in a saline solution and in a food matrix. Moreover, through microscope observations it has been possible to determine that the attractive binding forces between the positive amine corona on the surface of nanoparticles and the negatively charged bacteria membrane provoke a disruption of the cell membrane. The surface concentration of amines on the surface of the nanoparticles is so effective that immobilized-amines were 100 times more effective in killing L. monocytogenes bacteria than the same amount of free polyamines. This novel approach for the creation of antimicrobial nanodevices opens the possibility to put in value the antimicrobial power of natural molecules that have been discarded because of its low antimicrobial power. PRACTICAL APPLICATION Consumers demand for high-quality products, free from chemical preservatives, with an extended shelf-life. In this study, a really powerful antimicrobial agent based on a nanomaterial functionalized with a non-antimicrobial organic molecule was developed as a proof of concept. Following this approach it could be possible to develop a new generation of natural and removable antimicrobials based on their anchoring to functional surfaces for food, agricultural or medical purposes.

中文翻译：

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通过纳米技术提高低效抗菌分子的抗菌能力

这项工作的目的一方面是评估固定在介孔二氧化硅颗粒外表面的胺与相同剂量的游离胺相比对单核细胞增生李斯特菌的抗菌活性。我们的目标也是阐明新抗菌装置的作用机制。二氧化硅纳米粒子对锚定、浓缩和提高多胺对单核细胞增生李斯特菌的抗菌能力的适用性已在盐溶液和食品基质中得到证实。此外，通过显微镜观察，可以确定纳米颗粒表面上的正胺电晕与带负电的细菌膜之间的吸引力结合力会引起细胞膜的破坏。纳米颗粒表面胺的表面浓度非常有效，以至于固定胺在杀死单核细胞增生李斯特菌方面的效率是相同数量的游离多胺的 100 倍。这种用于创建抗菌纳米装置的新方法为评估由于其低抗菌能力而被丢弃的天然分子的抗菌能力提供了可能性。实际应用 消费者需要高质量的产品，不含化学防腐剂，保质期更长。在这项研究中，开发了一种非常强大的抗菌剂，该抗菌剂基于用非抗菌有机分子功能化的纳米材料，作为概念验证。